Force-mediated adhesion strengthening in endothelial cells at adherens junctions

Cells respond to the application of force with a variety of biochemical responses modulating their shape, structure, function, and proliferation. Two force-responsive links between the inside and outside of a cell are integrin proteins, which link a cell to the extracellular matrix (ECM), and cadherin proteins, which link neighboring cells to each other. The strength of integrin-ECM bonds has been noted to increase in response to the application of force. However, the strengthening of cadherin-cadherin bonds in response to force has not been studied. Here, we use magnetic trapping to probe adhesion strengthening at cadherin adherens junctions, using cadherin-coated magnetic beads to simulate neighboring cells and apply force at adherens junctions. 43% of beads exposed to a high force (2.1 nN) detached, compared to 31% of those exposed to a low-to-high force ramp followed by high force. This indicates that adherens junctions are strengthened by force application. The actin cytoskeleton and vasodilator-stimulated phosphoprotein (VASP) both associate with adherens junctions, so their role in adhesion strengthening at adherens junctions was also studied. Cells treated with actin-inhibitor cytochalasin D showed no difference in bead detachment from constant high force and from ramped followed by high force, indicating that the actin cytoskeleton is crucial in the adhesion strengthening response. Beads attached to cells expressing GFP-VASP, which behave like VASP-overexpressing cells, detached in 24% of trials when exposed to constant high force, compared to 39% of trials in response to ramped force. Cells expressing GFP-MITO-FPPPP, which behave like VASP-downregulated cells, showed no difference in bead detachment between application of high force and ramped force followed by high force.

Department dc:contributor.department

Massachusetts Institute of Technology. Biological Engineering Division.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2007

Author dc:creator

• Kris, Anita S

Advisor dc:contributor.advisor

• Roger D. Kamm.

• Biological Engineering Division.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/42386

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/42386