Comparison of the growth and monoclonal antibody production of suspended mammalian cells in three perfusion systems

The purpose of this thesis was to provide a broad survey of bioprocess options for typical drug production vehicles in the biotechnology industry. This goal was accomplished by comparing the growth and monoclonal antibody production by HPCHO Chinese hamster ovary cells and IB4 hybridoma cells in batch, fed-batch, and three perfusion systems: the stirred ceramic membrane reactor (SCMR), the alternating tangential flow (ATF) hollow fiber membrane system, and the external spin filter (ESF) system. The batch experiments for each cell line were used as base case scenarios for the comparisons of cell growth and monoclonal antibody production. The fed-batch experiments for each cell line did not vastly improve the cell growth and monoclonal antibody production over the batch cases. The SCMR perfusion system greatly improved the cell growth and monoclonal antibody production for each cell line. The maximum viable cell concentration of the IB4 hybridoma cells in the SCMR experiment was over seven times that found in the batch experiment and the monoclonal antibody production in the SCMR experiment was over ten times that found in the batch experiment.

Department dc:contributor.department

Massachusetts Institute of Technology. Biological Engineering Division.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2007

Author dc:creator

• Hufford, Kathy (Kathy E.)

Advisor dc:contributor.advisor

• Jean-Francois Hamel.

• Biological Engineering Division.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/39921

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/39921