Genetic engineering of bacteriophage and its applications for biomimetic materials

Filamentous bacteriophage (M13) are excellent biological build block due to their multiple peptide display system including type 8 (complete peptide display at pVIII) and type 83 (complete peptide display at both pVIII and pIII) display systems. Unlike the phagemid systems, the advantage of these systems is that we can get homogenous peptide display on pVIII resulting in uniform placement of selected molecules as well as defined length and width. In this thesis, type 8 and type 83 phage were constructed and used as biological scaffolds to meet the following four specific aims. First, the self-assembly of engineered M13 bacteriophage as a template for Co-Pt crystals was demonstrated. An phage library with an octapeptide library on the major coat protein (pVIII) was used for selection of binders to cobalt ions. Fibrous structures with directionally ordered phage were obtained by interaction with cobalt ions. Co-Pt alloys were synthesized on the fibrous scaffold, and their magnetic properties were characterized. The mineralization showed organized nanoparticles on fibrous bundles with superparamagnetic properties. Second, an in vitro molecular selection method in non-biological conditions for inorganic synthesis was introduced.

Department dc:contributor.department

Massachusetts Institute of Technology. Biological Engineering Division.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2006

Author dc:creator

• Lee, Soo-Kwan

Advisor dc:contributor.advisor

• Angela M. Belcher.

• Biological Engineering Division.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/38241

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/38241