A combined microfluidic/dielectrophoretic microorganism concentrator

This thesis presents the development of a high-throughput microfluidic microorganism concentrator for pathogen detection applications. Interdigitated electrodes lining the bottom of the channel use positive dielectrophoretic forces to trap particles. A passive mixer is used to circulate the liquid in the channel, transporting particles to within the trapping region of the electrodes. Samples are extracted and their concentrations measured using a spectrophotometer. Concentration enhancements up to 40x at 500 gl/min flowrate using polystyrene microspheres and up to 10x at 100 gll/min flowrate using B. subtilis spores are achieved.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2005

Author dc:creator

• Gadish, Nitzan

Advisor dc:contributor.advisor

• Joel Voldman.

• Electrical Engineering and Computer Science.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/36758

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/36758