Operations capability improvement of a molecular biology laboratory in a high throughput genome sequencing center

The Broad Institute is a research collaboration of MIT, Harvard University and affiliated hospitals, and the Whitehead Institute for Biomedical Research. Its scientific mission is to "(1) create tools for genomic medicine and make them broadly available to the scientific community and (2) apply these tools to propel the understanding and treatment of disease." The Broad Institute contains the world's largest high throughput genome sequencing center, which contributed approximately one third of the sequence for the Human Genome Project (HGP) completed in 2003. The Molecular Biology Production Group (MBPG) is the most upstream part of the Broad Institute's genome sequencing operation. This group is responsible for incoming DNA quality control, construction of DNA Libraries, and production of agar plates containing E.coli cell colonies (with many of copies of DNA). In this way, MPBG scales up raw DNA to a quality and quantity necessary for the subsequent high-volume, automated genome sequencing process. While most of the genome sequencing process at the Broad Institute had already been highly industrialized, MBPG had not yet undergone such a transformation and was still operated more like a laboratory than a manufacturing group.

Department dc:contributor.department

Leaders for Manufacturing Program at MIT

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2005

Author dc:creator

• Vokoun, Matthew R. (Matthew Richard)

Advisor dc:contributor.advisor

• Jeremie Gallien.

• Sloan School of Management.
• Chemical Engineering.
• Leaders for Manufacturing Program.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/35697

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/35697