Assembly and functionalization of phage onto substrates patterned by dip-pen nanolithography

Advances in nanochemistry will drive the development of technologies at the scale of 1 - 100 nm. Principles of biology are used for the self-assembly of structures and devices at this scale. The Ml 13 bacteriophage, a virus employed in phage-display libraries, serves as a scaffold for nanoscale structures. Phage are functionalized with inorganic materials, and controlled placement of phage at the nanoscale may lead to useful devices. Substrates patterned with dip-pen nanolithography (DPN) serve as templates for the deposition of phage. On gold substrates, 16-mercaptohexadecanoic acid (MHA) is deposited to form patterned lines. After surface passivation and activation chemistry, phage are deposited and adhere to the patterned substrate. Images from atomic force microscopy support that phage are covalently coupled to MHA lines and that cobalt precipitates on patterned phage.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Materials Science and Engineering.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2006

Author dc:creator

• Gray, David Steven

Advisor dc:contributor.advisor

• Angela M. Belcher.

• Materials Science and Engineering.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/35069

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/35069