Do heavy ions induce the bystander effect? : study to determine the induction of the bystander effect from Fe ion beam compared to X-rays in human keratinocytes

The bystander effect is the observation that non-irradiated cells near a cell traversed by radiation express biological responses such as micronuclei formation and genomic instability. Most published studies of the bystander effect have been conducted using alpha particles, a high LET radiation. A few studies have been done with low LET radiation. This project studies the bystander effect from both low LET x-rays and high LET Fe particle beam irradiation. Using a transwell insert system, the bystander effect was studied in hTERT immortalized human keratinocytes. Cells are plated in a 6-well plate and in a companion permeable membrane insert. Cells in the 6-well plate are irradiated using conventional 250 kVp X-rays or 1000 MeV/nucleon Fe ion beam, LET of 151 keV/pm, from the NASA Space Radiation Laboratory at Brookhaven National Lab. After irradiation, inserts are immediately placed into the 6 well plate so that the irradiated and unirradiated cells are sharing medium but are not in contact. For both beams, frequency of micronuclei, chromatin bridges, and p21 wafl induction as well as cell cycle phase analysis were determined in both directly irradiated and bystander cells from 0.1 Gy to 5 Gy. From x-rays, a two-fold bystander effect at 24 h after irradiation with elevated p21' wafl induction and micronuclei was seen but in Fe ion irradiation, the p21 wafl bystander effect was delayed to 40-50 h after irradiation and no MN bystander effect was observed.

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Nuclear Engineering

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2005

Author dc:creator

• Anzenberg, Vered

Advisor dc:contributor.advisor

• Kathryn D. Held.

• Nuclear Engineering.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/34458

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/34458