Direct and indirect effects of alpha-particle irradiations of human prostate tumor cells

The objective of this project is to establish a model system to study the direct effect, the bystander effect and the combinational effect of alpha-particle irradiations of human prostate tumor cells, toward the goal of improving the effectiveness of alpha-particle involved radiation therapies such as radioimmunotherapy and BNCT. This project consists of an engineering part and a biological part. In the engineering part, an apparatus for alpha-particle irradiation of cells was designed and constructed. Dosimetry of this irradiator was conducted. The average dose rate to the cell monolayer in the specially designed Mylar dish was calculated to be 1.2 Gy/min. In the biological part, three in vitro studies were carried out with the DU-145 human prostate tumor cells using the established alpha-particle irradiator. Firstly, the direct effects of the alpha-particle irradiation of DU-145 cells were studied using the clonogenic assay, the comet assay and the micronucleus assay. A novel co-culture system was then designed to study the bystander effect transmitted through the cell culture medium. The micronucleus assay was used as endpoint for the bystander studies. A bystander effect was observed.

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Nuclear Engineering

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2005

Author dc:creator

• Wang, Rong, Ph. D. Massachusetts Institute of Technology

Advisor dc:contributor.advisor

• Jeffrey A. Coderre.

• Nuclear Engineering.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/34439

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/34439