Characterization and optimization of PDMS microfluidic devices for rapid DNA hybridization

Two elastomeric microfluidic devices were designed for the purpose of conducting rapid, flow-based, multiplexed DNA hybridization. Experimental results showed that flowing hybridization assays could detect similar concentrations of labeled probe as standard stationary microarrays, but in 1/100h of the time, using 2% of the sample volume. An 8-channel device was used to spot glass slides with 64 hybridization assays and generate data supporting a theoretical model of DNA hybridization in both traditional stationary microarrays and flowing sample arrays. Larger devices were also used to create rrays of 96x96 spots on a single slide, demonstrating the scalability of the technology. Protocols were written and optimized for the use of both chips, allowing the technology to be distributed to collaborating labs for further development.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Mechanical Engineering.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2005

Author dc:creator

• Hu, Jenny (Jenny Ezu)

Advisor dc:contributor.advisor

• Todd Thorsen.

• Mechanical Engineering.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/32936

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/32936