Peripheral nerve regeneration through collagen devices with different in vivo degradation characteristics

In the United States more than 200,000 people are treated each year for peripheral nerve injuries that require surgery. Functional recovery of motor and sensory capability is limited following autograft, the most common procedure for peripheral nerve repair. Tubulation repair of a transected peripheral nerve presents an alternative to autograft, but has not yet shown the ability to satisfactorily restore lost function. A variety of degradable and non-degradable materials have been used to fabricate the tubes used to bridge the stumps of a transected nerve, and collagen tubes have been specifically shown to improve the quality of regeneration. The optimal characteristics for the tube have yet to be identified, and a collagen device that can be manipulated and optimized to improve functional peripheral nerve recovery is needed. The overall goal of this thesis was to fabricate and characterize a new collagen tube such that the tube characteristics and their effects on peripheral nerve regeneration can be closely studied, manipulated, and optimized. Following characterization of a homologous series of collagen tubes, each produced with a different density of crosslinks, the regenerative capacity of each member of the series of tubes was studied to determine the effects of device permeability, crosslink density, and in vivo tube degradation rate on peripheral nerve regeneration. It was first observed that the device permeability to cells and large soluble regulators significantly affected the quality of peripheral nerve regeneration, with the more permeable device (pore diameter 80 - 100 tm)

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Mechanical Engineering.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2002

Author dc:creator

• Harley, Brendan A. (Brendan Andrew), 1978-

Advisor dc:contributor.advisor

• Ioannis V. Yannas.

• Mechanical Engineering.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/32261

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/32261