Glycosaminoglycan biosynthesis controls C. elegans vulval morphogenesis and is required for the first cell division during embryogenesis

Morphogenesis is critical to the development of multicellular organisms. Morphogenesis is also a complex process that requires the coordination of many events, including cell division, cytoskeletal reorganization, cell-cell and cell-matrix interactions. To investigate a driving mechanism of morphogenesis in organ formation, we characterized a group of C. elegans mutants defective in vulval morphogenesis. We established that the morphogenetic defect is caused by disruption in biosynthesis of chondroitin glycosaminoglycans by molecular identification and characterization of five sqv (uashed vulva) genes. Glycosaminoglycans are known to function in development by binding a large repertoire of ligands involved in morphogenesis and wound healing, in some cases by modulating certain conserved signaling pathways, and in providing structural support in the extracellular matrix. Defects in biosynthesis of glycosaminoglycans can cause connective tissue diseases. We found that regulation of sqv-4 likely controls glycosaminoglycan biosynthesis and the aspect of vulval morphogenesis driven by chondroitin. We also characterized specific defects in separation of the plasma membrane from the eggshell and failure to initiate cytokinesis caused by mutations in sqv genes in the one-cell embryo.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Biology.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2002

Author dc:creator

• Hwang, Ho-Yon, 1969-

Advisor dc:contributor.advisor

• H. Robert Horvitz.

• Biology.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/29740

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/29740