Snapshots of radical enzyme catalysis : the crystal structures of biotin synthase and lysine 5,6-aminomutase

(cont.) in the same pathway. To further probe the relationship of the adenosylmethionine and adenosylcobalamin-dependent radical enzymes, the crystal structure of lysine 5,6-aminomutase was determined to 2.8 [angstroms] resolution. Like biotin synthase, the structure of lysine-5,6-aminomutase adheres to the general theme of barrels or barrel-like structures for enzymes that utilize 5'-deoxyadenosyl radicals in catalysis. Additionally, the structure of lysine-5,6-aminomutase implicates pyridoxal phosphate in a novel role, acting as a lock to prevent the formation of aberrant radicals in the absence of substrate.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Chemistry.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2004

Author dc:creator

• Berkovitch, Frederick, 1978-

Advisor dc:contributor.advisor

• Catherine L. Drennan.

• Chemistry.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

en_US

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/28698

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/28698