{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/28623"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/28623","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"Biological detection by means of mass reduction in a suspended microchannel resonator","abstract":"Label-free detection is the detection of biomolecules and their interactions, without the use of a molecule external to the interaction, used as a reporter to indicate presence and/or location. The suspended microchannel resonator offers the opportunity to perform such label-free measurements. The goal of this work is to open new avenues of possible applications for the suspended channel. I introduce the concept of detecting mass subtraction as a new approach, rather than the conventional detection of mass addition. In a model implementation scenario of this approach, a mass-intensifying tag bound to a small ligand molecule will be equilibrated with surface-immobilized receptors, and later displaced by an identical, but label-free, ligand molecule. This approach offers opportunities to extend the sensitivity range of the device, as well as introduces new functionality for it. It enables researchers to follow, label-free, real-time enzymatic reactions, relative affinities of different ligands to a receptor, and presence of small molecules in a solution.","abstract_html":"Label-free detection is the detection of biomolecules and their interactions, without the use of a molecule external to the interaction, used as a reporter to indicate presence and/or location. The suspended microchannel resonator offers the opportunity to perform such label-free measurements. The goal of this work is to open new avenues of possible applications for the suspended channel. I introduce the concept of detecting mass subtraction as a new approach, rather than the conventional detection of mass addition. In a model implementation scenario of this approach, a mass-intensifying tag bound to a small ligand molecule will be equilibrated with surface-immobilized receptors, and later displaced by an identical, but label-free, ligand molecule. This approach offers opportunities to extend the sensitivity range of the device, as well as introduces new functionality for it. It enables researchers to follow, label-free, real-time enzymatic reactions, relative affinities of different ligands to a receptor, and presence of small molecules in a solution.","abstract_has_math":false,"creators":["Levy-Tzedek, Shelly"],"institution":"Massachusetts Institute of Technology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. 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