{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/155575"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/155575","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"Vitamin A and glycoproteins of rat corneal epithelium","abstract":"Previous work from this laboratory has shown that the uptake of labeled precursors into a single specific glycopeptide in the rat intestinal mucosa was depressed significantly in vitamin A deficiency (De Luca et al ., 1970). A very similar pattern to the intestinal glycopeptide pattern was obtained from the rat corneal epithelium; namely, the glycopeptide affected by vitamin A was eluted with 0.4 N LiCl solution by a stepwise column chromatography on DEAE-Sephadex A-50. A specific \"peak S\" glycopeptide eluted between 0.35 N and 0.42 N LiCl solution was found to be the most decreased (approximately by 50%) glycopeptide component by vitamin A deficiency when a new continuous gradient column chromatography of the same anion exchanger was employed. \"Peak S\" glycopeptide was further characterized by polyacrylamide (7.5%) gel electrophoresis and gas-liquid chromatography. The affected \"peak S\" glycopeptide was found to be rich in sialic acid, possibly as an end-sugar. Topical application for one hour in vivo of water-dispersible vitamin A palmitate to corneas of deficient rats resulted in a stimulation of glycoprotein/glycopeptide synthesis upon subsequent incubation in vitro. Upon fractionation of glycopeptide prepared from vitamin A-deficient control and deficient, vitamin A-treated rat corneal epithelium by DEAE-Sephadex A-50 column chromatography, the fraction eluted in 0.2 N LiCl solution showed a marked increase in labeling of 14c-glucosamine into glycopeptides. This stimulated fraction appears to consist of small molecules probably lacking sialic acid. The in vitro stimulation of glycopeptide synthesis in vitamin A-deficient corneal epithelium seems to be confined also to the 0.2 N LiCl fraction. Histologically, corneal epithelium, and particularly the mucus-secreting conjunctival gland showed a strong fluorescent response to fluorescent antibody made against the intestinal glycopeptide affected by vitamin A.","abstract_html":"Previous work from this laboratory has shown that the uptake of labeled precursors into a single specific glycopeptide in the rat intestinal mucosa was depressed significantly in vitamin A deficiency (De Luca et al ., 1970). A very similar pattern to the intestinal glycopeptide pattern was obtained from the rat corneal epithelium; namely, the glycopeptide affected by vitamin A was eluted with 0.4 N LiCl solution by a stepwise column chromatography on DEAE-Sephadex A-50. A specific "peak S" glycopeptide eluted between 0.35 N and 0.42 N LiCl solution was found to be the most decreased (approximately by 50%) glycopeptide component by vitamin A deficiency when a new continuous gradient column chromatography of the same anion exchanger was employed. "Peak S" glycopeptide was further characterized by polyacrylamide (7.5%) gel electrophoresis and gas-liquid chromatography. The affected "peak S" glycopeptide was found to be rich in sialic acid, possibly as an end-sugar. Topical application for one hour in vivo of water-dispersible vitamin A palmitate to corneas of deficient rats resulted in a stimulation of glycoprotein/glycopeptide synthesis upon subsequent incubation in vitro. Upon fractionation of glycopeptide prepared from vitamin A-deficient control and deficient, vitamin A-treated rat corneal epithelium by DEAE-Sephadex A-50 column chromatography, the fraction eluted in 0.2 N LiCl solution showed a marked increase in labeling of 14c-glucosamine into glycopeptides. This stimulated fraction appears to consist of small molecules probably lacking sialic acid. The in vitro stimulation of glycopeptide synthesis in vitamin A-deficient corneal epithelium seems to be confined also to the 0.2 N LiCl fraction. Histologically, corneal epithelium, and particularly the mucus-secreting conjunctival gland showed a strong fluorescent response to fluorescent antibody made against the intestinal glycopeptide affected by vitamin A.","abstract_has_math":false,"creators":["Yang, Cha Lee Kim."],"institution":"Massachusetts Institute of Technology","degree_name":"Doctoral","degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. Department of Nutrition and Food Science","school":null,"contributors":[],"advisors":["George Wolf."],"committee_chairs":[],"committee_members":[],"year":1973,"date_issued":"1973","date_published":"1973","updated_at":"2026-07-22T22:21:41Z","subjects":["Nutrition and Food Science."],"languages":["eng"],"rights":["MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided."],"rights_urls":["http://dspace.mit.edu/handle/1721.1/7582"],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/1721.1/155575","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["George Wolf."]},{"key":"dc:contributor.department","label":"Department","values":["Massachusetts Institute of Technology. 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Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided."]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://dspace.mit.edu/handle/1721.1/7582"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/1721.1/155575"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Thesis: Ph. D., Massachusetts Institute of Technology, Department of Nutrition and Food Science, 1973","Cataloged from PDF of print version of thesis. Vita.","Includes bibliographical references (pages 174-184)."]},{"key":"dc:description.abstract","label":"Abstract","values":["Previous work from this laboratory has shown that the uptake of labeled precursors into a single specific glycopeptide in the rat intestinal mucosa was depressed significantly in vitamin A deficiency (De Luca et al ., 1970). A very similar pattern to the intestinal glycopeptide pattern was obtained from the rat corneal epithelium; namely, the glycopeptide affected by vitamin A was eluted with 0.4 N LiCl solution by a stepwise column chromatography on DEAE-Sephadex A-50. A specific \"peak S\" glycopeptide eluted between 0.35 N and 0.42 N LiCl solution was found to be the most decreased (approximately by 50%) glycopeptide component by vitamin A deficiency when a new continuous gradient column chromatography of the same anion exchanger was employed. \"Peak S\" glycopeptide was further characterized by polyacrylamide (7.5%) gel electrophoresis and gas-liquid chromatography. The affected \"peak S\" glycopeptide was found to be rich in sialic acid, possibly as an end-sugar. Topical application for one hour in vivo of water-dispersible vitamin A palmitate to corneas of deficient rats resulted in a stimulation of glycoprotein/glycopeptide synthesis upon subsequent incubation in vitro. Upon fractionation of glycopeptide prepared from vitamin A-deficient control and deficient, vitamin A-treated rat corneal epithelium by DEAE-Sephadex A-50 column chromatography, the fraction eluted in 0.2 N LiCl solution showed a marked increase in labeling of 14c-glucosamine into glycopeptides. This stimulated fraction appears to consist of small molecules probably lacking sialic acid. The in vitro stimulation of glycopeptide synthesis in vitamin A-deficient corneal epithelium seems to be confined also to the 0.2 N LiCl fraction. Histologically, corneal epithelium, and particularly the mucus-secreting conjunctival gland showed a strong fluorescent response to fluorescent antibody made against the intestinal glycopeptide affected by vitamin A."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph. D."]},{"key":"dc:title","label":"Title","values":["Vitamin A and glycoproteins of rat corneal epithelium"]}]}],"canonical_facts":{"dc:contributor.advisor":["George Wolf."],"dc:contributor.department":["Massachusetts Institute of Technology. Department of Nutrition and Food Science"],"dc:contributor.other":["Massachusetts Institute of Technology. Department of Nutrition and Food Science."],"dc:creator":["Yang, Cha Lee Kim."],"dc:date.accessioned":["2024-07-10T17:38:13Z"],"dc:date.available":["2024-07-10T17:38:13Z"],"dc:date.issued":["1973"],"dc:description":["Thesis: Ph. D., Massachusetts Institute of Technology, Department of Nutrition and Food Science, 1973","Cataloged from PDF of print version of thesis. Vita.","Includes bibliographical references (pages 174-184)."],"dc:description.abstract":["Previous work from this laboratory has shown that the uptake of labeled precursors into a single specific glycopeptide in the rat intestinal mucosa was depressed significantly in vitamin A deficiency (De Luca et al ., 1970). A very similar pattern to the intestinal glycopeptide pattern was obtained from the rat corneal epithelium; namely, the glycopeptide affected by vitamin A was eluted with 0.4 N LiCl solution by a stepwise column chromatography on DEAE-Sephadex A-50. A specific \"peak S\" glycopeptide eluted between 0.35 N and 0.42 N LiCl solution was found to be the most decreased (approximately by 50%) glycopeptide component by vitamin A deficiency when a new continuous gradient column chromatography of the same anion exchanger was employed. \"Peak S\" glycopeptide was further characterized by polyacrylamide (7.5%) gel electrophoresis and gas-liquid chromatography. The affected \"peak S\" glycopeptide was found to be rich in sialic acid, possibly as an end-sugar. Topical application for one hour in vivo of water-dispersible vitamin A palmitate to corneas of deficient rats resulted in a stimulation of glycoprotein/glycopeptide synthesis upon subsequent incubation in vitro. Upon fractionation of glycopeptide prepared from vitamin A-deficient control and deficient, vitamin A-treated rat corneal epithelium by DEAE-Sephadex A-50 column chromatography, the fraction eluted in 0.2 N LiCl solution showed a marked increase in labeling of 14c-glucosamine into glycopeptides. This stimulated fraction appears to consist of small molecules probably lacking sialic acid. The in vitro stimulation of glycopeptide synthesis in vitamin A-deficient corneal epithelium seems to be confined also to the 0.2 N LiCl fraction. Histologically, corneal epithelium, and particularly the mucus-secreting conjunctival gland showed a strong fluorescent response to fluorescent antibody made against the intestinal glycopeptide affected by vitamin A."],"dc:description.degree":["Ph. D."],"dc:identifier.uri":["https://hdl.handle.net/1721.1/155575"],"dc:language.iso":["eng"],"dc:publisher":["Massachusetts Institute of Technology"],"dc:rights":["MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided."],"dc:rights.uri":["http://dspace.mit.edu/handle/1721.1/7582"],"dc:subject":["Nutrition and Food Science."],"dc:title":["Vitamin A and glycoproteins of rat corneal epithelium"],"dc:type":["Academic theses.","Thesis"],"thesis:degree_name":["Doctoral"]},"updated_at":"2026-07-22T22:21:41Z"}