Designing Novel DNA-Binding Proteins with Generative Deep Learning

Protein-DNA interactions play a critical role in various biological processes, such as gene regulation and genome maintenance. Designing protein backbones specifically tailored for DNA binding remains a challenging task, requiring the exploration of novel computational approaches. This thesis presents a novel framework for gen- erating protein backbones that exhibit affinity for DNA molecules. The proposed methodology leverages Graph Neural Networks (GNNs) for encoding protein struc- tures and diffusion models for conditional sampling. The GNNs capture the intricate relationships between amino acids in the protein backbone, allowing for the effective encoding of structural information relevant to DNA binding. The diffusion models enable the conditional generation of protein backbones, given specific DNA sequences as input. The thesis proposes a Transformer architecture and provides a practical way to diffuse from its protein encoding. The findings from this research have significant implications for the design and engineering of DNA binding proteins, facilitating ad- vancements in fields such as synthetic biology, gene therapy, and drug development.

Name thesis:degree_name

Master

Department dc:contributor.department

Program in Media Arts and Sciences (Massachusetts Institute of Technology)

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2023

Author dc:creator

• Calman, Ido

Advisor dc:contributor.advisor

• Jacobson, Joseph

Statement dc:rights

• In Copyright - Educational Use Permitted
• Copyright retained by author(s)

Licence dc:rights.uri

• https://rightsstatements.org/page/InC-EDU/1.0/

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/152003

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/152003