{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/146677"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/146677","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"Integrated Microfluidic Culture Media Oxygenator for Organ-on-a-Chip Applications","abstract":"Microphysiological systems (MPS) are in vitro platforms for the culture of human cells in a manner that closely mimics the in vivo physiological microenvironment. Oxygen is a key element for maintaining cell viability and function in MPS devices. Thus, this thesis presents the design, fabrication, and testing of a microfluidic oxygenator chip, or \"Oxychip\", for providing control of oxygen concentration in MPS cell culture media. The oxygenation mechanism of the device features a serpentine fluidic channel that contacts a pneumatic pocket through a gas permeable membrane, to allow for oxygen exchange between the liquid media and the gas in the pneumatic compartment. Additionally, the Oxychip integrates the oxygenator module with an on-board micropump, pressure regulator, culture media reservoir, and oxygen probe interface, in a compact 40 x 25 mm microfluidic chip. To validate the functionality of the device, a series of three experiments were conducted in which oxygen partial pressure within circulating fluid media was monitored using oxygen probes, and rapid deoxygenation and re-oxygenation of fluid media was demonstrated.","abstract_html":"Microphysiological systems (MPS) are in vitro platforms for the culture of human cells in a manner that closely mimics the in vivo physiological microenvironment. Oxygen is a key element for maintaining cell viability and function in MPS devices. Thus, this thesis presents the design, fabrication, and testing of a microfluidic oxygenator chip, or "Oxychip", for providing control of oxygen concentration in MPS cell culture media. The oxygenation mechanism of the device features a serpentine fluidic channel that contacts a pneumatic pocket through a gas permeable membrane, to allow for oxygen exchange between the liquid media and the gas in the pneumatic compartment. Additionally, the Oxychip integrates the oxygenator module with an on-board micropump, pressure regulator, culture media reservoir, and oxygen probe interface, in a compact 40 x 25 mm microfluidic chip. To validate the functionality of the device, a series of three experiments were conducted in which oxygen partial pressure within circulating fluid media was monitored using oxygen probes, and rapid deoxygenation and re-oxygenation of fluid media was demonstrated.","abstract_has_math":false,"creators":["Dey Barsukova, Anita"],"institution":"Massachusetts Institute of Technology","degree_name":"Bachelor","degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. Department of Mechanical Engineering","school":null,"contributors":[],"advisors":["David L. Trumper"],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-05","date_published":"2022-05","updated_at":"2026-07-22T22:21:59Z","subjects":[],"languages":[],"rights":["In Copyright - Educational Use Permitted","Copyright MIT"],"rights_urls":["http://rightsstatements.org/page/InC-EDU/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/1721.1/146677","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["David L. Trumper"]},{"key":"dc:contributor.department","label":"Department","values":["Massachusetts Institute of Technology. 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Oxygen is a key element for maintaining cell viability and function in MPS devices. Thus, this thesis presents the design, fabrication, and testing of a microfluidic oxygenator chip, or \"Oxychip\", for providing control of oxygen concentration in MPS cell culture media. The oxygenation mechanism of the device features a serpentine fluidic channel that contacts a pneumatic pocket through a gas permeable membrane, to allow for oxygen exchange between the liquid media and the gas in the pneumatic compartment. Additionally, the Oxychip integrates the oxygenator module with an on-board micropump, pressure regulator, culture media reservoir, and oxygen probe interface, in a compact 40 x 25 mm microfluidic chip. To validate the functionality of the device, a series of three experiments were conducted in which oxygen partial pressure within circulating fluid media was monitored using oxygen probes, and rapid deoxygenation and re-oxygenation of fluid media was demonstrated."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["S.B."]},{"key":"dc:title","label":"Title","values":["Integrated Microfluidic Culture Media Oxygenator for Organ-on-a-Chip Applications"]}]}],"canonical_facts":{"dc:contributor.advisor":["David L. Trumper"],"dc:contributor.department":["Massachusetts Institute of Technology. Department of Mechanical Engineering"],"dc:creator":["Dey Barsukova, Anita"],"dc:date.accessioned":["2022-11-30T19:40:48Z"],"dc:date.available":["2022-11-30T19:40:48Z"],"dc:date.issued":["2022-05"],"dc:description.abstract":["Microphysiological systems (MPS) are in vitro platforms for the culture of human cells in a manner that closely mimics the in vivo physiological microenvironment. Oxygen is a key element for maintaining cell viability and function in MPS devices. Thus, this thesis presents the design, fabrication, and testing of a microfluidic oxygenator chip, or \"Oxychip\", for providing control of oxygen concentration in MPS cell culture media. The oxygenation mechanism of the device features a serpentine fluidic channel that contacts a pneumatic pocket through a gas permeable membrane, to allow for oxygen exchange between the liquid media and the gas in the pneumatic compartment. Additionally, the Oxychip integrates the oxygenator module with an on-board micropump, pressure regulator, culture media reservoir, and oxygen probe interface, in a compact 40 x 25 mm microfluidic chip. To validate the functionality of the device, a series of three experiments were conducted in which oxygen partial pressure within circulating fluid media was monitored using oxygen probes, and rapid deoxygenation and re-oxygenation of fluid media was demonstrated."],"dc:description.degree":["S.B."],"dc:identifier.uri":["https://hdl.handle.net/1721.1/146677"],"dc:publisher":["Massachusetts Institute of Technology"],"dc:rights":["In Copyright - Educational Use Permitted","Copyright MIT"],"dc:rights.uri":["http://rightsstatements.org/page/InC-EDU/1.0/"],"dc:title":["Integrated Microfluidic Culture Media Oxygenator for Organ-on-a-Chip Applications"],"dc:type":["Thesis"],"thesis:degree_name":["Bachelor","Bachelor of Science in Mechanical Engineering"]},"updated_at":"2026-07-22T22:21:59Z"}