Non-Parametric Analyses of the Regulatory Roles of LINE-1 Retrotransposons during Motor Neuron Differentiation

Background: Repetitive elements make up a large portion of eukaryotic genomes, constituting two-thirds of human genome. Although their functional importance were recognized as early as 1950s, study of their functions is stagnated despite the advancements in next-generation sequencing due to difficulty in establishing the identities of specific elements involved in a biological process of interest, e.g. transcription factor (TF) binding, from functional data modalities such as ChIP-seq and ChIA-PET. Results: First, I present a non-parametric, k-mer based method that overcomes analysis ambiguities introduced by short read multimapping and the incompleteness of reference genomes in low-complexity regions. I use this method to elucidate inferential evidence for the cell type-specific binding of transcription factors to specific L1 subfamilies from ChIP-seq datasets. Second, I applied a method named Mates of Chimera (MoC) to identify L1-derived extrachromosomal circular DNAs (eccDNAs) from Circulome-seq datasets. I characterized differential eccDNA compositions in ESC and MN cell types and found differential enrichment of transcription factor binding motifs in cell type specific eccDNAs. Third, I present inferential evidence consistent with the hypothesis that some low-complexity regions may participate in chromatin interactions with cis-regulatory sequences in a cell-type specific manner analogous to enhancer-promoter interactions. Conclusion: The thesis elucidates a set of functional hypotheses concerning putative regulatory roles of repetitive elements, L1 elements in particular, that may be extrachromosomal. I base my hypotheses on a wide range of available data modalities including whole-genome sequencing, ChIP-seq, Circulome-seq, and ChIA-PET through non-parametric, k-mer based methods that do not rely on exact read alignment coordinates.

Name thesis:degree_name

Master

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2022

Author dc:creator

• Park, Hyunjin

Advisor dc:contributor.advisor

• Gifford, David K.

Statement dc:rights

• In Copyright - Educational Use Permitted
• Copyright MIT

Licence dc:rights.uri

• http://rightsstatements.org/page/InC-EDU/1.0/

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/143310

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/143310