{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/140073"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/140073","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device","abstract":"Biological assays for various biological samples are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific labeling and result in a purified sample with a higher purity of target particles, enabling a higher chance of detecting/analyzing those particles of interest for biological assays. Moreover, the cell-sorting ability of spiral devices can be applied as a cell-washing technology to isolate target cells while removing unwanted particles such as adventitious agents for a better quality of cellular products or manufacturing cells in biomanufacturing. This work provides numerous applications of spiral cell sorter-based microfluidic sample preparation on various biological samples. We propose to apply spiral microfluidic sorter for various biological samples to acquire enhanced readouts for targeted downstream assays such as Next-generation sequencing (NGS) or improved quality of cells for biomanufacturing.","abstract_html":"Biological assays for various biological samples are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific labeling and result in a purified sample with a higher purity of target particles, enabling a higher chance of detecting/analyzing those particles of interest for biological assays. Moreover, the cell-sorting ability of spiral devices can be applied as a cell-washing technology to isolate target cells while removing unwanted particles such as adventitious agents for a better quality of cellular products or manufacturing cells in biomanufacturing. This work provides numerous applications of spiral cell sorter-based microfluidic sample preparation on various biological samples. We propose to apply spiral microfluidic sorter for various biological samples to acquire enhanced readouts for targeted downstream assays such as Next-generation sequencing (NGS) or improved quality of cells for biomanufacturing.","abstract_has_math":false,"creators":["Choi, Kyungyong"],"institution":"Massachusetts Institute of Technology","degree_name":"Doctoral","degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science","school":null,"contributors":[],"advisors":["Han, Jongyoon"],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-09","date_published":"2021-09","updated_at":"2026-07-22T22:21:51Z","subjects":[],"languages":[],"rights":["In Copyright - Educational Use Permitted","Copyright MIT"],"rights_urls":["http://rightsstatements.org/page/InC-EDU/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/1721.1/140073","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Han, Jongyoon"]},{"key":"dc:contributor.department","label":"Department","values":["Massachusetts Institute of Technology. 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A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific labeling and result in a purified sample with a higher purity of target particles, enabling a higher chance of detecting/analyzing those particles of interest for biological assays. Moreover, the cell-sorting ability of spiral devices can be applied as a cell-washing technology to isolate target cells while removing unwanted particles such as adventitious agents for a better quality of cellular products or manufacturing cells in biomanufacturing. This work provides numerous applications of spiral cell sorter-based microfluidic sample preparation on various biological samples. We propose to apply spiral microfluidic sorter for various biological samples to acquire enhanced readouts for targeted downstream assays such as Next-generation sequencing (NGS) or improved quality of cells for biomanufacturing."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph.D."]},{"key":"dc:title","label":"Title","values":["Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device"]}]}],"canonical_facts":{"dc:contributor.advisor":["Han, Jongyoon"],"dc:contributor.department":["Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science"],"dc:creator":["Choi, Kyungyong"],"dc:date.accessioned":["2022-02-07T15:22:29Z"],"dc:date.available":["2022-02-07T15:22:29Z"],"dc:date.issued":["2021-09"],"dc:description.abstract":["Biological assays for various biological samples are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific labeling and result in a purified sample with a higher purity of target particles, enabling a higher chance of detecting/analyzing those particles of interest for biological assays. Moreover, the cell-sorting ability of spiral devices can be applied as a cell-washing technology to isolate target cells while removing unwanted particles such as adventitious agents for a better quality of cellular products or manufacturing cells in biomanufacturing. This work provides numerous applications of spiral cell sorter-based microfluidic sample preparation on various biological samples. We propose to apply spiral microfluidic sorter for various biological samples to acquire enhanced readouts for targeted downstream assays such as Next-generation sequencing (NGS) or improved quality of cells for biomanufacturing."],"dc:description.degree":["Ph.D."],"dc:identifier.uri":["https://hdl.handle.net/1721.1/140073"],"dc:publisher":["Massachusetts Institute of Technology"],"dc:rights":["In Copyright - Educational Use Permitted","Copyright MIT"],"dc:rights.uri":["http://rightsstatements.org/page/InC-EDU/1.0/"],"dc:title":["Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device"],"dc:type":["Thesis"],"thesis:degree_name":["Doctoral","Doctor of Philosophy"]},"updated_at":"2026-07-22T22:21:51Z"}