{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/139951"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/139951","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"Interactions between mobile genetic elements and their bacterial hosts","abstract":"Integrative and conjugative elements (ICEs) are widespread mobile genetic elements that facilitate the spread of many important genes, including those involved in antibiotic resistance, metabolism, pathogenesis, or symbiosis. These elements are also powerful tools for genetic analyses and engineering. ICEs are typically found integrated in a host chromosome. Either stochastically, or upon some signal, they can excise, undergo DNA processing events, be transferred through encoded conjugation machinery into a neighboring cell, and stably integrate into the new chromosome. Interactions between an ICE and its hosts throughout the life cycle can influence the efficiency of acquisition by new hosts. In this work, I investigated and compared the interactions that two ICEs, Tn916 and ICEBs1, have with their host cells. First, I explored how the different functional modules of these elements impact how efficiently they are transferred into different host species. I generated hybrid conjugative elements that merge functions of both elements to increase transfer efficiencies, presenting exciting potential to be used for genetic engineering. Next, I investigated a previously unknown ability of Tn916 to cause a growth arrest and kill its host cell. I took genetic approaches to determine that two Tn916-encoded genes interact with a defective phage-like element in the B. subtilis chromosome to elicit some of these effects. Finally, I evaluated the integration site selection of Tn916 in the B. subtilis chromosome, identifying several hundred unique AT-rich insertion sites, one of which is a “hot spot” for integration. I found that a host nucleoid-associated protein does not influence integration site selection. I conclude this body of work with a discussion of how the efficient spread of an element is shaped by its interactions with host cells and other horizontally acquired elements.","abstract_html":"Integrative and conjugative elements (ICEs) are widespread mobile genetic elements that facilitate the spread of many important genes, including those involved in antibiotic resistance, metabolism, pathogenesis, or symbiosis. These elements are also powerful tools for genetic analyses and engineering. ICEs are typically found integrated in a host chromosome. Either stochastically, or upon some signal, they can excise, undergo DNA processing events, be transferred through encoded conjugation machinery into a neighboring cell, and stably integrate into the new chromosome. Interactions between an ICE and its hosts throughout the life cycle can influence the efficiency of acquisition by new hosts. In this work, I investigated and compared the interactions that two ICEs, Tn916 and ICEBs1, have with their host cells. First, I explored how the different functional modules of these elements impact how efficiently they are transferred into different host species. I generated hybrid conjugative elements that merge functions of both elements to increase transfer efficiencies, presenting exciting potential to be used for genetic engineering. Next, I investigated a previously unknown ability of Tn916 to cause a growth arrest and kill its host cell. I took genetic approaches to determine that two Tn916-encoded genes interact with a defective phage-like element in the B. subtilis chromosome to elicit some of these effects. Finally, I evaluated the integration site selection of Tn916 in the B. subtilis chromosome, identifying several hundred unique AT-rich insertion sites, one of which is a “hot spot” for integration. I found that a host nucleoid-associated protein does not influence integration site selection. I conclude this body of work with a discussion of how the efficient spread of an element is shaped by its interactions with host cells and other horizontally acquired elements.","abstract_has_math":false,"creators":["Clark, Emily L."],"institution":"Massachusetts Institute of Technology","degree_name":"Doctoral","degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. Microbiology Graduate Program","school":null,"contributors":[],"advisors":["Grossman, Alan D."],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-09","date_published":"2021-09","updated_at":"2026-07-22T22:21:41Z","subjects":[],"languages":[],"rights":["In Copyright - Educational Use Permitted","Copyright MIT"],"rights_urls":["http://rightsstatements.org/page/InC-EDU/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/1721.1/139951","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Grossman, Alan D."]},{"key":"dc:contributor.department","label":"Department","values":["Massachusetts Institute of Technology. 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These elements are also powerful tools for genetic analyses and engineering. ICEs are typically found integrated in a host chromosome. Either stochastically, or upon some signal, they can excise, undergo DNA processing events, be transferred through encoded conjugation machinery into a neighboring cell, and stably integrate into the new chromosome. Interactions between an ICE and its hosts throughout the life cycle can influence the efficiency of acquisition by new hosts. In this work, I investigated and compared the interactions that two ICEs, Tn916 and ICEBs1, have with their host cells. First, I explored how the different functional modules of these elements impact how efficiently they are transferred into different host species. I generated hybrid conjugative elements that merge functions of both elements to increase transfer efficiencies, presenting exciting potential to be used for genetic engineering. Next, I investigated a previously unknown ability of Tn916 to cause a growth arrest and kill its host cell. I took genetic approaches to determine that two Tn916-encoded genes interact with a defective phage-like element in the B. subtilis chromosome to elicit some of these effects. Finally, I evaluated the integration site selection of Tn916 in the B. subtilis chromosome, identifying several hundred unique AT-rich insertion sites, one of which is a “hot spot” for integration. I found that a host nucleoid-associated protein does not influence integration site selection. I conclude this body of work with a discussion of how the efficient spread of an element is shaped by its interactions with host cells and other horizontally acquired elements."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph.D."]},{"key":"dc:title","label":"Title","values":["Interactions between mobile genetic elements and their bacterial hosts"]}]}],"canonical_facts":{"dc:contributor.advisor":["Grossman, Alan D."],"dc:contributor.department":["Massachusetts Institute of Technology. Microbiology Graduate Program"],"dc:creator":["Clark, Emily L."],"dc:date.accessioned":["2022-02-07T15:15:11Z"],"dc:date.available":["2022-02-07T15:15:11Z"],"dc:date.issued":["2021-09"],"dc:description.abstract":["Integrative and conjugative elements (ICEs) are widespread mobile genetic elements that facilitate the spread of many important genes, including those involved in antibiotic resistance, metabolism, pathogenesis, or symbiosis. These elements are also powerful tools for genetic analyses and engineering. ICEs are typically found integrated in a host chromosome. Either stochastically, or upon some signal, they can excise, undergo DNA processing events, be transferred through encoded conjugation machinery into a neighboring cell, and stably integrate into the new chromosome. Interactions between an ICE and its hosts throughout the life cycle can influence the efficiency of acquisition by new hosts. In this work, I investigated and compared the interactions that two ICEs, Tn916 and ICEBs1, have with their host cells. First, I explored how the different functional modules of these elements impact how efficiently they are transferred into different host species. I generated hybrid conjugative elements that merge functions of both elements to increase transfer efficiencies, presenting exciting potential to be used for genetic engineering. Next, I investigated a previously unknown ability of Tn916 to cause a growth arrest and kill its host cell. I took genetic approaches to determine that two Tn916-encoded genes interact with a defective phage-like element in the B. subtilis chromosome to elicit some of these effects. Finally, I evaluated the integration site selection of Tn916 in the B. subtilis chromosome, identifying several hundred unique AT-rich insertion sites, one of which is a “hot spot” for integration. I found that a host nucleoid-associated protein does not influence integration site selection. I conclude this body of work with a discussion of how the efficient spread of an element is shaped by its interactions with host cells and other horizontally acquired elements."],"dc:description.degree":["Ph.D."],"dc:identifier.uri":["https://hdl.handle.net/1721.1/139951"],"dc:publisher":["Massachusetts Institute of Technology"],"dc:rights":["In Copyright - Educational Use Permitted","Copyright MIT"],"dc:rights.uri":["http://rightsstatements.org/page/InC-EDU/1.0/"],"dc:title":["Interactions between mobile genetic elements and their bacterial hosts"],"dc:type":["Thesis"],"thesis:degree_name":["Doctoral","Doctor of Philosophy"]},"updated_at":"2026-07-22T22:21:41Z"}