Protein immobilization using complex coacervates and complex coacervate thin films

Enzymes can enable a wide and growing range of chemistries, often outperforming synthetic catalysts. However, enzymes must often be converted to heterogeneous catalysts. Protein immobilization enables this conversion and can enhance the stability of enzymes. Complex coacervates are highly effective at encapsulating and stabilizing enzymes. This thesis demonstrates the use of complex coacervate thin films for the immobilization of enzymes and systematically probes methods to enhance the performance of these materials. The first study presents a proof-of-concept demonstration of complex coacervate thin films for the synthesis of functional biomaterials. The immobilization method itself is all-aqueous, reducing the likelihood of enzyme denaturation, and facile, only requiring two steps: coating followed by crosslinking.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Chemical Engineering

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2021

Author dc:creator

• Sureka, Hursh Vardhan.

Advisor dc:contributor.advisor

• Bradley D. Olsen.

• Chemical Engineering.

Statement dc:rights

• MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/130824

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/130824