Engineering non-immunoglobulin binding proteins for in vitro diagnostic Tests

In 2016, nearly 5.5 million deaths were attributed to infectious and parasitic diseases. Although many of these diseases are preventable and treatable, resource-constrained regions often lack access to rapid and accurate diagnostic tests to appropriately diagnose and treat these diseases. In order to improve the accessibility of diagnostics, the development of low-cost, simple, and rapid diagnostic tests is vital. Antibodies have been widely used as the binding reagents in these tests to detect a target biomarker from the patient sample. These tests are often designed as a sandwich assay, which requires a pair of antibodies as complementary capture and reporter reagents. However, antibodies have some limitations for use in in vitro applications, including variable stability from clone to clone, long developmental timelines, and structural complexity.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Chemical Engineering

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2020

Author dc:creator

• Sung, Ki-Joo.

Advisor dc:contributor.advisor

• Hadley D. Sikes.

• Chemical Engineering.

Statement dc:rights

• MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/130192

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/130192