A general method for three color STED microscopy with one depletion laser : application to primary neuronal culture

Recent advances in the field of optical nanoscopy have equipped molecular neuroscientists with the means to investigate sub-synaptic phenomena with unprecedented spatial resolution. Indeed, early studies employing these methodologies have revealed an extraordinary level of nanoscale spatiotemporal organization of complex and highly dynamic molecular architectures at the pre- and postsynaptic compartments. How exactly these supramolecular complexes are modified through time and experience, though, remains largely unknown. Moreover, the mechanisms by which pre- and postsynaptic protein organization specifies the biochemical and electrophysiological properties of a synapse have yet to be adequately described. The purpose of the current study is thus to develop an inexpensive, accessible platform for super-resolution microscopy (SRM) of synaptic nanostructure.

Name thesis:degree_name

Master

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2020

Author dc:creator

• Lee, Mackenzie C.,S.M.Massachusetts Institute of Technology.

Advisor dc:contributor.advisor

• Rebecca Saxe.

• Brain and Cognitive Sciences.

Statement dc:rights

• MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/127884

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/127884