Enabling Cl-Based bioconversion with metabolic engineering

Single-carbon (C) substrates, such as synthesis gas and methanol, are attractive feedstocks for biochemical processes, as they are widely available, can be produced renewably, and do not compete with food supply. However, their use in industrial bioprocessing remains limited, primarily because microbes that utilize these substrates are poorly characterized biochemically, and limited tools exist for their genetic modification. This leaves the metabolic engineer with a choice: to develop genetic tools to enable engineering in the desired host, or to import the relevant catabolic pathway into a more tractable organism, such as Escherichia coli. This thesis explores both options within the context of developing strains for the conversion of C1 substrates into value-added chemicals and fuels.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Chemical Engineering

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2017

Author dc:creator

• Woolston, Benjamin Michael.

Advisor dc:contributor.advisor

• Gregory Stephanopoulos.

• Chemical Engineering.

Statement dc:rights

• MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/127713

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/127713