{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/124238"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/124238","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"Integrated multiparametric deep spatial phenotyping of mouse models of lung adenocarcinoma","abstract":"In this thesis, I developed computational pipelines and algorithms that use high dimensional biomarker imaging data to predict features of tumor tissues taken from a genetically engineered mouse model (GEMM) of lung adenocarcinoma. I extracted biomarker expression levels and morphological, textural, and spatial motifs of single cells from the imaging data and used these features to train algorithms to predict tumor histologic grade, a measure correlated with the malignant potential of a tumor. The algorithm predictions were evaluated through comparison to a validated deep learning model. The random forest algorithm achieved a 72% accuracy classifying cells as belonging to a non-tumor, grade 1, grade 2, or grade 3 region and achieved a 87% accuracy classifying cells as belonging to a tumor or non-tumor region. A combination of biomarker, morphological, textural, and spatial features generated models that performed better than any single group of markers by itself; spatial features in particular significantly improved model performance.","abstract_html":"In this thesis, I developed computational pipelines and algorithms that use high dimensional biomarker imaging data to predict features of tumor tissues taken from a genetically engineered mouse model (GEMM) of lung adenocarcinoma. I extracted biomarker expression levels and morphological, textural, and spatial motifs of single cells from the imaging data and used these features to train algorithms to predict tumor histologic grade, a measure correlated with the malignant potential of a tumor. The algorithm predictions were evaluated through comparison to a validated deep learning model. The random forest algorithm achieved a 72% accuracy classifying cells as belonging to a non-tumor, grade 1, grade 2, or grade 3 region and achieved a 87% accuracy classifying cells as belonging to a tumor or non-tumor region. A combination of biomarker, morphological, textural, and spatial features generated models that performed better than any single group of markers by itself; spatial features in particular significantly improved model performance.","abstract_has_math":false,"creators":["Dai, Yang,M. Eng.Massachusetts Institute of Technology."],"institution":"Massachusetts Institute of Technology","degree_name":"Master","degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science","school":null,"contributors":[],"advisors":["Sandro Santagata and Tyler Jacks."],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019","date_published":"2019","updated_at":"2026-07-22T22:22:20Z","subjects":["Electrical Engineering and Computer Science."],"languages":["eng"],"rights":["MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission."],"rights_urls":["http://dspace.mit.edu/handle/1721.1/7582"],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/1721.1/124238","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Sandro Santagata and Tyler Jacks."]},{"key":"dc:contributor.department","label":"Department","values":["Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science","EECS"]},{"key":"dc:contributor.other","label":"Dc Contributor Other","values":["Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science."]},{"key":"dc:creator","label":"Author","values":["Dai, Yang,M. 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They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission."]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://dspace.mit.edu/handle/1721.1/7582"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/1721.1/124238"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.","Thesis: M. 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The random forest algorithm achieved a 72% accuracy classifying cells as belonging to a non-tumor, grade 1, grade 2, or grade 3 region and achieved a 87% accuracy classifying cells as belonging to a tumor or non-tumor region. A combination of biomarker, morphological, textural, and spatial features generated models that performed better than any single group of markers by itself; spatial features in particular significantly improved model performance."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["M. Eng."]},{"key":"dc:title","label":"Title","values":["Integrated multiparametric deep spatial phenotyping of mouse models of lung adenocarcinoma"]}]}],"canonical_facts":{"dc:contributor.advisor":["Sandro Santagata and Tyler Jacks."],"dc:contributor.department":["Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science","EECS"],"dc:contributor.other":["Massachusetts Institute of Technology. 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