Solid-state NMR investigation of viral fusion glycoprotein 41 (gp41)

Protein-mediated membrane fusion is an integral part of numerous cellular processes and viral entry. The HIV envelope glycoprotein mediates viral entry into target cells by fusing the viral envelope with the cell membrane. This process requires large-scale and multi-step conformational changes of the viral fusion protein gp41. Our current understanding of the mechanisms of protein-induced membrane structural changes involved in viral entry is incomplete because the hydrophobic N-terminal fusion peptide (FP) and C-terminal transmembrane domain (TMD) of gp41 have resisted structure determination. In our study, we expressed a gp41 construct, "short NC", containing both hydrophobic termini, including the FP, the fusion-peptide proximal region (FPPR), the membrane-proximal external region (MPER), and the TMD, together with a truncated water-soluble ectodomain linking the N and C termini. In order to probe the membrane-bound topology and conformation of gp41, we reconstituted "short NC" gp41 into a virus-mimetic membrane for solid-state NMR experiments. ¹³C chemical shifts of ¹³C isotopically labeled residues indicated that the C-terminal MPER-TMD region is predominantly [alpha]-helical, whereas the N-terminal FP-FPPR exhibits [beta]-sheet character. Water and lipid ¹H polarization transfer to the protein revealed that the TMD is well inserted into the membrane, while the FPPR and MPER are more hydrated and exposed to the membrane surface. Importantly, we observed correlation signals between the FP-FPPR and the MPER, providing direct evidence that the ectodomain is sufficiently collapsed to bring the N- and C-terminal hydrophobic domains into close proximity. These results support a hemifusion-like structural model of gp41 in which its ectodomain forms a partially folded hairpin that places the FPPR and MPER on the opposing surfaces of two lipid membranes that are in close proximity.

Name thesis:degree_name

Master

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Chemistry

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2019

Author dc:creator

• Morgan, Chloe A.(Chloe Anne)

Advisor dc:contributor.advisor

• Mei Hong.

• Chemistry.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/124056

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/124056