Glycoprotein mimetic materials - synthetic methods and study of viral inhibition properties

The human body houses some of the most unique materials on the planet and is a promising source of bioinspiration. The high molecular weight and glycosylation of mucin-type glycopeptides make them challenging to produce or even mimic. This material production challenge is one of the key limiting factors for the study and design of biological materials such as mucus and cartilage. The first section of this thesis develops two approaches for creating high molecular weight heavily modified protein brushes. The first uses a combination of cysteine and diazo coupling reactions, while the second uses combination of global amino acid substitution and copper()-catalyzed alkyne-azide cycloaddition (CuAAC). The tyrosine enrichment required to prepare elastin-like-polypeptides (ELP) proteins for diazo coupling reactions prevented protein expression at 96 pentapeptide repeats and above, but maintained yields comparable to other work at 48 repeats and below.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Chemical Engineering

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2019

Author dc:creator

• Seifried, Brian(Brian Michael)

Advisor dc:contributor.advisor

• Bradley D. Olsen.

• Chemical Engineering.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/124042

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/124042