Alternatively spliced isoforms of Fibronectin, Tenascin-R and other potential players in early vasculogenesis

The absence of both the EIIIA and EIIIB domains of fibronectin (FN) has been shown to negatively affect blood vessel formation and maintenance. Vascular defects have been observed in the yolk sacs of EIIIA/B double-null embryos by as early as embryonic day E9.5, and these defects are likely due to alterations in the extracellular matrix (ECM). Therefore, I have conducted this study to investigate the differences in the ECM composition of the yolk sac in the presence and absence of EIIIA and EIIIB. I first collected yolk sacs at E9.5 from wild type, EIIIA-null, EIIIB-null, EIIIA/B heterozygous and EIIIA/B double-null mouse embryos, enriched for ECM content, and used quantitative proteomics to analyze their ECM composition. From these data, we identified a set of matrisome proteins that had decreased abundance in EIIIA/B double-null yolk sacs but were relatively unchanged in single-null and heterozygous yolk sacs compared to wild type.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Biology

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2019

Author dc:creator

• Nguyen, Thao Huong.

Advisor dc:contributor.advisor

• Richard 0. Hynes.

• Biology.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/123718

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/123718