Engineering protein-based modulators of allergic, temporal, and checkpoint blockade anti-cancer immunity

Effective cancer treatment of the future requires incorporating diverse and innovative aspects of immunity to fight against cancer, accounting for pharmacokinetic and temporal barriers of therapeutics, and engineering approaches to understand and improve upon current immunotherapies. This thesis addresses these challenges in three projects utilizing the Wittrup Lab's quantitative, engineering approach to protein-based cancer immunotherapy. In the first project, I attempted to harness the potency of allergic reactions against cancer by designing IgE class antibodies against two mouse tumor antigens and comparing them with traditional IgG antibodies. These IgE antibodies elicited modest or no tumor control, and limited efficacy could be due to fast pharmacokinetic clearance, absence of human-like allergic effector cells in mice, or tumor-suppressive effects from mast cells responding to IgE.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Biological Engineering

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2019

Author dc:creator

• Rothschilds, Adrienne Marie.

Advisor dc:contributor.advisor

• K. Dane Wittrup.

• Biological Engineering.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/123064

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/123064