Methodologies for the mechanistic study of compartment-specific hydrogen peroxide perturbations

Reactive oxygen species (ROS) are an interesting class of molecules because of their ability to promote contradictory phenotypes depending on their intracellular concentration. Most significantly, their elevation has been linked with several pathologies, including cancer. The selective cancer killing hypothesis hinges on the idea that certain cancers will be more susceptible to toxicity via a redox-based mechanism than their surrounding healthy counterparts, and provides an attractive target for those studying redox biology. In order to effectively leverage this strategy, quantitative knowledge of intracellular ROS, specifically hydrogen peroxide (H₂O₂) and its associated pathway proteins, is necessary. This thesis developed tools and methodology for quantitative and mechanistic studies of H₂O₂ in the mitochondria.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Chemical Engineering

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2019

Author dc:creator

• Stein, Kassi Taylor.

Advisor dc:contributor.advisor

• Hadley D. Sikes.

• Chemical Engineering.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/122906

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/122906