Sequence design principles for 3D wireframe DNA origami

DNA is a highly programmable molecule that can be designed to self-assemble into nearly arbitrary 2D and 3D nanoscale structures. DNA origami is a particularly versatile method to achieve complex molecular architectures. However, the rules for designing scaffolded DNA origami have not been well-formalized, which hinders both the investigation of characteristics of well- and poorly-folded structures as well as the participation of a larger scientific audience in DNA nanotechnology. In my thesis work, a fully automatic inverse design procedure DAEDALUS (DNA Origami Sequence Design Algorithm for User-defined Structures) has been developed that programs arbitrary wireframe DNA assemblies based on an input wireframe mesh without reliance on user feedback. This general, top-down strategy is able to design nearly arbitrary DNA architectures, routing the scaffold strand using a spanning tree algorithm and adding staple strands in a prescribed manner. The wireframe nanoparticles produced can use antiparallel crossover (DX) motifs, for robust selfassembly, parallel paranemic crossover (PX) motifs, for staple-free self-assembly, or a hybrid of the two, to minimize the number of staples required for folding to the ones necessary for functionalization. The thermodynamics of the self-assembly of these wireframe structures, and the effects of scaffold and staple routing, are investigated using quantitative PCR and FRET measurements, tracking fluorescence to elucidate global and local folding events. The framework developed should enable the broad participation of nonexperts in this powerful molecular design paradigm and set the foundation for further predictive models of DNA self-assembly.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Chemical Engineering

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2018

Author dc:creator

• Ratanalert, Sakul.

Advisor dc:contributor.advisor

• Mark Bathe and Gregory C. Rutledge.

• Chemical Engineering.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/121818

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/121818