{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/119713"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/119713","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"High-throughput multiplexed transcriptome and protein measurements in single cells","abstract":"We present cell gels, a platform for high-throughput multiplexed measurements of transcriptome and proteome in single cells. This method takes advantage of droplet barcoding and hydrogel chemistry to capture mRNAs and proteins in thousands of cells. We report the challenges of acquiring sequencing data from cell gels due to complex mechanisms underlying bead based library prep in polyacrylamide gels. 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