{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/118286"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/118286","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"Critical role of histone tail entropy in nucleosome unwinding","abstract":"As the fundamental packaging unit for the genome, the nucleosome is of central importance for many essential biological processes and has been the focus of numerous research efforts. The dynamics of the nucleosome is of particular interest as it must be balanced to maintain long-lasting genome stability while keeping the DNA accessible to protein molecules. Using a transferable protein-DNA model and advanced sampling techniques, we investigated the stability and dynamics of the nucleosome by determining the free energy cost of its DNA unwinding. Simulation results quantitatively reproduce thermodynamic parameters estimated from single-molecule force spectroscopy experiments, and capture the appearance of a large energetic barrier as the system transitions from the outer to the inner layer of DNA unwinding. Analysis of partially unwound nucleosome configurations at atomic resolution revealed that the transition barrier arises from a delayed loss of contacts between histone tails and the DNA. Surprisingly, there is a significant entropic contribution from the same set of disordered tails that largely offset the energetic barrier. Our study greatly improves the current understanding of nucleosome unwinding by providing detailed mechanistic insights into experimental observations.","abstract_html":"As the fundamental packaging unit for the genome, the nucleosome is of central importance for many essential biological processes and has been the focus of numerous research efforts. The dynamics of the nucleosome is of particular interest as it must be balanced to maintain long-lasting genome stability while keeping the DNA accessible to protein molecules. Using a transferable protein-DNA model and advanced sampling techniques, we investigated the stability and dynamics of the nucleosome by determining the free energy cost of its DNA unwinding. Simulation results quantitatively reproduce thermodynamic parameters estimated from single-molecule force spectroscopy experiments, and capture the appearance of a large energetic barrier as the system transitions from the outer to the inner layer of DNA unwinding. Analysis of partially unwound nucleosome configurations at atomic resolution revealed that the transition barrier arises from a delayed loss of contacts between histone tails and the DNA. Surprisingly, there is a significant entropic contribution from the same set of disordered tails that largely offset the energetic barrier. Our study greatly improves the current understanding of nucleosome unwinding by providing detailed mechanistic insights into experimental observations.","abstract_has_math":false,"creators":["Parsons, Thomas T. (Thomas Tyler)"],"institution":"Massachusetts Institute of Technology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. Department of Chemistry.","school":null,"contributors":[],"advisors":["Bin Zhang."],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018","date_published":"2018","updated_at":"2026-07-22T22:22:29Z","subjects":["Chemistry."],"languages":["eng"],"rights":["MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission."],"rights_urls":["http://dspace.mit.edu/handle/1721.1/7582"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/1721.1/118286","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Bin Zhang."]},{"key":"dc:contributor.department","label":"Department","values":["Massachusetts Institute of Technology. Department of Chemistry."]},{"key":"dc:contributor.other","label":"Dc Contributor Other","values":["Massachusetts Institute of Technology. Department of Chemistry."]},{"key":"dc:creator","label":"Author","values":["Parsons, Thomas T. (Thomas Tyler)"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2018-09-28T21:00:18Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2018-09-28T21:00:18Z"]},{"key":"dc:date.issued","label":"Date","values":["2018"]},{"key":"dc:publisher","label":"Institution","values":["Massachusetts Institute of Technology"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission."]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://dspace.mit.edu/handle/1721.1/7582"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/1721.1/118286"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Thesis: S.M., Massachusetts Institute of Technology, Department of Chemistry, 2018.","Cataloged from PDF version of thesis.","Includes bibliographical references (pages 33-36)."]},{"key":"dc:description.abstract","label":"Abstract","values":["As the fundamental packaging unit for the genome, the nucleosome is of central importance for many essential biological processes and has been the focus of numerous research efforts. The dynamics of the nucleosome is of particular interest as it must be balanced to maintain long-lasting genome stability while keeping the DNA accessible to protein molecules. Using a transferable protein-DNA model and advanced sampling techniques, we investigated the stability and dynamics of the nucleosome by determining the free energy cost of its DNA unwinding. Simulation results quantitatively reproduce thermodynamic parameters estimated from single-molecule force spectroscopy experiments, and capture the appearance of a large energetic barrier as the system transitions from the outer to the inner layer of DNA unwinding. Analysis of partially unwound nucleosome configurations at atomic resolution revealed that the transition barrier arises from a delayed loss of contacts between histone tails and the DNA. Surprisingly, there is a significant entropic contribution from the same set of disordered tails that largely offset the energetic barrier. Our study greatly improves the current understanding of nucleosome unwinding by providing detailed mechanistic insights into experimental observations."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["S.M."]},{"key":"dc:title","label":"Title","values":["Critical role of histone tail entropy in nucleosome unwinding"]}]}],"canonical_facts":{"dc:contributor.advisor":["Bin Zhang."],"dc:contributor.department":["Massachusetts Institute of Technology. Department of Chemistry."],"dc:contributor.other":["Massachusetts Institute of Technology. Department of Chemistry."],"dc:creator":["Parsons, Thomas T. (Thomas Tyler)"],"dc:date.accessioned":["2018-09-28T21:00:18Z"],"dc:date.available":["2018-09-28T21:00:18Z"],"dc:date.issued":["2018"],"dc:description":["Thesis: S.M., Massachusetts Institute of Technology, Department of Chemistry, 2018.","Cataloged from PDF version of thesis.","Includes bibliographical references (pages 33-36)."],"dc:description.abstract":["As the fundamental packaging unit for the genome, the nucleosome is of central importance for many essential biological processes and has been the focus of numerous research efforts. The dynamics of the nucleosome is of particular interest as it must be balanced to maintain long-lasting genome stability while keeping the DNA accessible to protein molecules. Using a transferable protein-DNA model and advanced sampling techniques, we investigated the stability and dynamics of the nucleosome by determining the free energy cost of its DNA unwinding. Simulation results quantitatively reproduce thermodynamic parameters estimated from single-molecule force spectroscopy experiments, and capture the appearance of a large energetic barrier as the system transitions from the outer to the inner layer of DNA unwinding. Analysis of partially unwound nucleosome configurations at atomic resolution revealed that the transition barrier arises from a delayed loss of contacts between histone tails and the DNA. Surprisingly, there is a significant entropic contribution from the same set of disordered tails that largely offset the energetic barrier. Our study greatly improves the current understanding of nucleosome unwinding by providing detailed mechanistic insights into experimental observations."],"dc:description.degree":["S.M."],"dc:identifier.uri":["http://hdl.handle.net/1721.1/118286"],"dc:language.iso":["eng"],"dc:publisher":["Massachusetts Institute of Technology"],"dc:rights":["MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission."],"dc:rights.uri":["http://dspace.mit.edu/handle/1721.1/7582"],"dc:subject":["Chemistry."],"dc:title":["Critical role of histone tail entropy in nucleosome unwinding"],"dc:type":["Thesis"]},"updated_at":"2026-07-22T22:22:29Z"}