{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/112427"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/112427","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"Illuminating the function of a master regulator of Caulobacter crescentus gene expression","abstract":"Bacteria must allocate cellular resources required for transcription in a manner that accommodates the temporally regulated expression of functionally specialized gene products and the constitutive synthesis of stable RNAs required for ribosome biogenesis and translation. The Caulobacter crescentus cell cycle has been well-characterized and represents a powerful model system for studying the regulation of global gene expression patterns and \"just-in-time\" transcription. Here, we provide evidence that the Caulobacter GcrA protein directly regulates transcription at active promoters of highly expressed genes, particularly those encoding stable RNAs. Furthermore, we demonstrate that GcrA chromosome occupancy is dynamic and subject to epigenetic regulation. Our findings establish GcrA as an integral regulator of bacterial physiology and development.","abstract_html":"Bacteria must allocate cellular resources required for transcription in a manner that accommodates the temporally regulated expression of functionally specialized gene products and the constitutive synthesis of stable RNAs required for ribosome biogenesis and translation. The Caulobacter crescentus cell cycle has been well-characterized and represents a powerful model system for studying the regulation of global gene expression patterns and "just-in-time" transcription. Here, we provide evidence that the Caulobacter GcrA protein directly regulates transcription at active promoters of highly expressed genes, particularly those encoding stable RNAs. Furthermore, we demonstrate that GcrA chromosome occupancy is dynamic and subject to epigenetic regulation. Our findings establish GcrA as an integral regulator of bacterial physiology and development.","abstract_has_math":false,"creators":["Yuan, Andy H. (Andy Han)"],"institution":"Massachusetts Institute of Technology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. Department of Biology.","school":null,"contributors":[],"advisors":["Michael T. Laub."],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012","date_published":"2012","updated_at":"2026-07-22T22:21:24Z","subjects":["Biology."],"languages":["eng"],"rights":["MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission."],"rights_urls":["http://dspace.mit.edu/handle/1721.1/7582"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/1721.1/112427","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Michael T. Laub."]},{"key":"dc:contributor.department","label":"Department","values":["Massachusetts Institute of Technology. Department of Biology."]},{"key":"dc:contributor.other","label":"Dc Contributor Other","values":["Massachusetts Institute of Technology. Department of Biology."]},{"key":"dc:creator","label":"Author","values":["Yuan, Andy H. (Andy Han)"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-12-05T19:12:21Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2017-12-05T19:12:21Z"]},{"key":"dc:date.issued","label":"Date","values":["2012"]},{"key":"dc:publisher","label":"Institution","values":["Massachusetts Institute of Technology"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission."]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://dspace.mit.edu/handle/1721.1/7582"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/1721.1/112427"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Thesis: S.M., Massachusetts Institute of Technology, Department of Biology, 2012.","Cataloged from PDF version of thesis.","Includes bibliographical references (pages 25-27)."]},{"key":"dc:description.abstract","label":"Abstract","values":["Bacteria must allocate cellular resources required for transcription in a manner that accommodates the temporally regulated expression of functionally specialized gene products and the constitutive synthesis of stable RNAs required for ribosome biogenesis and translation. The Caulobacter crescentus cell cycle has been well-characterized and represents a powerful model system for studying the regulation of global gene expression patterns and \"just-in-time\" transcription. Here, we provide evidence that the Caulobacter GcrA protein directly regulates transcription at active promoters of highly expressed genes, particularly those encoding stable RNAs. Furthermore, we demonstrate that GcrA chromosome occupancy is dynamic and subject to epigenetic regulation. Our findings establish GcrA as an integral regulator of bacterial physiology and development."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["S.M."]},{"key":"dc:title","label":"Title","values":["Illuminating the function of a master regulator of Caulobacter crescentus gene expression"]}]}],"canonical_facts":{"dc:contributor.advisor":["Michael T. Laub."],"dc:contributor.department":["Massachusetts Institute of Technology. Department of Biology."],"dc:contributor.other":["Massachusetts Institute of Technology. Department of Biology."],"dc:creator":["Yuan, Andy H. (Andy Han)"],"dc:date.accessioned":["2017-12-05T19:12:21Z"],"dc:date.available":["2017-12-05T19:12:21Z"],"dc:date.issued":["2012"],"dc:description":["Thesis: S.M., Massachusetts Institute of Technology, Department of Biology, 2012.","Cataloged from PDF version of thesis.","Includes bibliographical references (pages 25-27)."],"dc:description.abstract":["Bacteria must allocate cellular resources required for transcription in a manner that accommodates the temporally regulated expression of functionally specialized gene products and the constitutive synthesis of stable RNAs required for ribosome biogenesis and translation. The Caulobacter crescentus cell cycle has been well-characterized and represents a powerful model system for studying the regulation of global gene expression patterns and \"just-in-time\" transcription. Here, we provide evidence that the Caulobacter GcrA protein directly regulates transcription at active promoters of highly expressed genes, particularly those encoding stable RNAs. Furthermore, we demonstrate that GcrA chromosome occupancy is dynamic and subject to epigenetic regulation. Our findings establish GcrA as an integral regulator of bacterial physiology and development."],"dc:description.degree":["S.M."],"dc:identifier.uri":["http://hdl.handle.net/1721.1/112427"],"dc:language.iso":["eng"],"dc:publisher":["Massachusetts Institute of Technology"],"dc:rights":["MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission."],"dc:rights.uri":["http://dspace.mit.edu/handle/1721.1/7582"],"dc:subject":["Biology."],"dc:title":["Illuminating the function of a master regulator of Caulobacter crescentus gene expression"],"dc:type":["Thesis"]},"updated_at":"2026-07-22T22:21:24Z"}