Fabrication of reinforced alginate fibers for cell based therapies

Type I Diabetes is a condition in which the pancreas does not produce enough insulin, leading to dysregulation in glucose metabolism. Most Type I diabetics take insulin injections after each meal in order to remain euglycemic. Transplantations of islet cells in immuno-protective alginate capsules has been shown to be promising as a long-term cure for Type I Diabetes. In this study, reinforced alginate fibers were fabricated as an alternative to alginate capsules for islet transplantation. Surgical thread was covered in alginate-cell solution by the microfluidic nozzle system to give a stronger structure to be used for implanting the cells. The thickness of the cell-encapsulated alginate layer produced by microfluidic nozzle processing system was controlled by varying the flow rate of alginate being pumped through the nozzle as well as by varying the speed by which the thread was extracted from the nozzle. The study showed that higher flow rates of alginate and lower thread speeds result in thicker alginate layers for encapsulating more cells within the fiber. The cells remained viable over a month, suggesting plausibility of cell-encapsulated microfibers as a long term solution to diabetes treatment.

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Mechanical Engineering.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2016

Author dc:creator

• Carter, Sydney C. (Sydney Cheyenne)

Advisor dc:contributor.advisor

• Robert Langer.

• Mechanical Engineering.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/110959

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/110959