Quantitative modeling for microbial ecology and clinical trials

Microbial ecology has benefited from the decreased cost and increased quality of next-generation DNA sequencing. In general, studies that use DNA sequencing are no longer limited by the sequencing itself but instead by the acquisition of the samples and by methods for analyzing and interpreting the resulting sequence data. In this thesis, I describe the results of three projects that address challenges to interpreting or acquiring sequence data. In the first project, I developed a method for analyzing the dynamics of the relative abundance of operational taxonomic units measured by next-generation amplicon sequencing in microbial ecology experiments without replication. In the second project, I and my co-author combined a taxonomic survey of a dimictic lake, an ecosystem-level biogeochemical model of microbial metabolisms in the lake, and the results of a single-cell genetic assay to infer the identity of taxonomically-diverse, putatively-syntrophic microbial consortia. In the third project, I and my co-author developed a model of differences in the efficacy that stool from different donors has when treating patients via fecal microbiota transplant. We use that model to compute statistical powers and to optimize clinical trial designs. Aside from contributing scientific conclusions about each system, these methods will also serve as a conceptual framework for future efforts to address challenges to the interpretation or acquisition of microbial ecology data.

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Biological Engineering.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2016

Author dc:creator

• Olesen, Scott Wilder

Advisor dc:contributor.advisor

• Eric J. Alm.

• Biological Engineering.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/107277

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/107277