{"id":{"repo_id":"missouri","oai_identifier":"oai:mospace.umsystem.edu:10355/7021"},"canonical_url":"https://search.dev.ndltd.org/etd/missouri/oai:mospace.umsystem.edu:10355/7021","repository":{"repo_id":"missouri","name":"University of Missouri","base_url":"https://mospace.umsystem.edu/oai/request"},"display":{"title":"Frequency domain fluorescent molecular tomography and molecular probes for small animal imaging","abstract":"In this thesis, we have developed a frequency domain fluorescent molecular tomographic system based on the heterodyne technique using a single source and detector pair for small animal imaging. In our system, the intensity of laser source is modulated to produce a diffuse photon density wave in the tissue. The phase of the diffuse photon density wave is measured by comparing the reference signal with the signal from tissue by using a phasemeter. In parallel, we have developed and evaluated fluorescent Alexa Fluor 680 and Alexa Fluor 750-Bombesin (BBN) probes to target gastrin-releasing peptide (GRP) receptors on prostate and breast cancer for optical molecular imaging. Confocal fluorescence microscopic imaging of the molecular probes for in vitro PC-3 prostate and T-47D breast cancer cell lines indicated specific uptake, internalization and receptor blocking of these probes. In vivo investigations in severely compromised immunodeficient (SCID) mice bearing xenografted PC-3 prostate and T47-D breast cancer lesions demonstrated the ability of this new molecular probes to specifically target tumor tissue with a high selectively and affinity.","abstract_html":"In this thesis, we have developed a frequency domain fluorescent molecular tomographic system based on the heterodyne technique using a single source and detector pair for small animal imaging. In our system, the intensity of laser source is modulated to produce a diffuse photon density wave in the tissue. The phase of the diffuse photon density wave is measured by comparing the reference signal with the signal from tissue by using a phasemeter. In parallel, we have developed and evaluated fluorescent Alexa Fluor 680 and Alexa Fluor 750-Bombesin (BBN) probes to target gastrin-releasing peptide (GRP) receptors on prostate and breast cancer for optical molecular imaging. Confocal fluorescence microscopic imaging of the molecular probes for in vitro PC-3 prostate and T-47D breast cancer cell lines indicated specific uptake, internalization and receptor blocking of these probes. In vivo investigations in severely compromised immunodeficient (SCID) mice bearing xenografted PC-3 prostate and T47-D breast cancer lesions demonstrated the ability of this new molecular probes to specifically target tumor tissue with a high selectively and affinity.","abstract_has_math":false,"creators":["Kujala, Naresh Gandhi, 1975-"],"institution":"University of Missouri--Columbia","degree_name":"Ph. 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In our system, the intensity of laser source is modulated to produce a diffuse photon density wave in the tissue. The phase of the diffuse photon density wave is measured by comparing the reference signal with the signal from tissue by using a phasemeter. In parallel, we have developed and evaluated fluorescent Alexa Fluor 680 and Alexa Fluor 750-Bombesin (BBN) probes to target gastrin-releasing peptide (GRP) receptors on prostate and breast cancer for optical molecular imaging. Confocal fluorescence microscopic imaging of the molecular probes for in vitro PC-3 prostate and T-47D breast cancer cell lines indicated specific uptake, internalization and receptor blocking of these probes. In vivo investigations in severely compromised immunodeficient (SCID) mice bearing xenografted PC-3 prostate and T47-D breast cancer lesions demonstrated the ability of this new molecular probes to specifically target tumor tissue with a high selectively and affinity."]},{"key":"dc:title","label":"Title","values":["Frequency domain fluorescent molecular tomography and molecular probes for small animal imaging"]}]}],"canonical_facts":{"dc:contributor.advisor":["Yu, Ping, 1962 December-"],"dc:creator":["Kujala, Naresh Gandhi, 1975-"],"dc:date.accessioned":["2010-04-27T14:34:54Z"],"dc:date.available":["2010-04-27T14:34:54Z"],"dc:date.issued":["2009"],"dc:description":["Title from PDF of title page (University of Missouri--Columbia, viewed on Feb 26, 2010).","The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.","Dissertation advisor: Dr. Ping Yu.","Vita.","Ph.D. University of Missouri--Columbia 2009."],"dc:description.abstract":["In this thesis, we have developed a frequency domain fluorescent molecular tomographic system based on the heterodyne technique using a single source and detector pair for small animal imaging. 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