Controlled loading of cryoprotectants (CPAS) with cell volume constraints
type: CreativeWork
description: [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI-COLUMBIA AT AUTHOR'S REQUEST.] Oocyte cryopreservation techniques are considered very important aspects in biomedical research for oocyte and embryo cryopreservation to be used in future in In Vitro Fertilization (IVF) procedure. Oocyte cryopreservation is an important aspect in many applications that involves deffering the time of pregnancy to future due to many reasons such as chemo therapy for the ones who have cancer or failed IVF cycles that reduce the cost for future cycles. The definition of optimal cryopreservation protocols highly depends on knowing the permeability of the cell membrane to water and cryoprotective agents. Due to the large size of oocytes that requires microscopy methods which could be difficult to implement, micro fluidic device is presented in this study based on Coulter-principle to simultaneously control and measure the volume of a single cell. In this thesis we designed and fabricated a micro fluidic device to monitor the cell volume change to allow the implementation of state-space optimized CPA equilibration strategies without prior knowledge of cell membrane transport parameters.
abstract: [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI-COLUMBIA AT AUTHOR'S REQUEST.] Oocyte cryopreservation techniques are considered very important aspects in biomedical research for oocyte and embryo cryopreservation to be used in future in In Vitro Fertilization (IVF) procedure. Oocyte cryopreservation is an important aspect in many applications that involves deffering the time of pregnancy to future due to many reasons such as chemo therapy for the ones who have cancer or failed IVF cycles that reduce the cost for future cycles. The definition of optimal cryopreservation protocols highly depends on knowing the permeability of the cell membrane to water and cryoprotective agents. Due to the large size of oocytes that requires microscopy methods which could be difficult to implement, micro fluidic device is presented in this study based on Coulter-principle to simultaneously control and measure the volume of a single cell. In this thesis we designed and fabricated a micro fluidic device to monitor the cell volume change to allow the implementation of state-space optimized CPA equilibration strategies without prior knowledge of cell membrane transport parameters.
author: Jasim, Ibrahem Hashim Mahmood
language: eng
language: English
date published: 2015
date modified: 2026-07-24T03:07:46Z
provider: University of Missouri
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Missouri--Columbia

PropertyValue
property ID: Handle
value: https://hdl.handle.net/10355/58499