{"id":{"repo_id":"missouri","oai_identifier":"oai:mospace.umsystem.edu:10355/5811"},"canonical_url":"https://search.dev.ndltd.org/etd/missouri/oai:mospace.umsystem.edu:10355/5811","repository":{"repo_id":"missouri","name":"University of Missouri","base_url":"https://mospace.umsystem.edu/oai/request"},"display":{"title":"Arachidonate 15-lipoxygenase and ubiquitin as potential fertility markers in boars","abstract":"[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] This study was performed to determine whether using high power microscopic analysis and two putative biochemical fertility markers, 15-lipoxygenase (15-LOX) and ubiquitin (UBI), could be used to accurately evaluate boar semen quality. In this study, ejaculates (n=116) were collected from 18 Large White boars for eight months (April, August-February). Ejaculates were analyzed and the percentage of proximal CDs (PCD), distal CDs (DCD), distal midpiece reflex CDs (DMR) and other spermatozoa abnormalities (ABN). Further ejaculate analysis was performed by semi-quantitative western blotting with computerized densitometry using antibodies against 15-LOX and UBI. Fertility data including FR and TNB was collected from sows mated to the boars in the study. Correlation coefficient analysis found moderate, negative relationships between FRs and DCDs (-0.39, P<0.01) and DMRs (-0.24, P<0.05), but not PCDs. These data suggest that an accurate boar semen analysis can be achieved by improved microscopy and by the use of biochemical fertility markers.","abstract_html":"[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR&#x27;S REQUEST.] This study was performed to determine whether using high power microscopic analysis and two putative biochemical fertility markers, 15-lipoxygenase (15-LOX) and ubiquitin (UBI), could be used to accurately evaluate boar semen quality. In this study, ejaculates (n=116) were collected from 18 Large White boars for eight months (April, August-February). Ejaculates were analyzed and the percentage of proximal CDs (PCD), distal CDs (DCD), distal midpiece reflex CDs (DMR) and other spermatozoa abnormalities (ABN). Further ejaculate analysis was performed by semi-quantitative western blotting with computerized densitometry using antibodies against 15-LOX and UBI. Fertility data including FR and TNB was collected from sows mated to the boars in the study. Correlation coefficient analysis found moderate, negative relationships between FRs and DCDs (-0.39, P&lt;0.01) and DMRs (-0.24, P&lt;0.05), but not PCDs. These data suggest that an accurate boar semen analysis can be achieved by improved microscopy and by the use of biochemical fertility markers.","abstract_has_math":false,"creators":["Lovercamp, Kyle W."],"institution":"University of Missouri--Columbia","degree_name":"M.A.","degree_level":"Masters","degree_discipline":"Animal sciences (MU)","degree_department":null,"school":null,"contributors":[],"advisors":["Safranski, Timothy Jon, 1965-","Sutovsky, Peter"],"committee_chairs":[],"committee_members":[],"year":2004,"date_issued":"2004","date_published":"2004","updated_at":"2026-07-24T03:08:43Z","subjects":[],"languages":["eng","English"],"rights":["Access to files is limited to the campuses of the University of Missouri with SSO login."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10355/5811","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Safranski, Timothy Jon, 1965-","Sutovsky, Peter"]},{"key":"dc:creator","label":"Author","values":["Lovercamp, Kyle W."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2010-02-23T19:52:39Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2010-02-23T19:52:39Z"]},{"key":"dc:date.issued","label":"Date","values":["2004"]},{"key":"dc:publisher","label":"Institution","values":["University of Missouri--Columbia"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal sciences (MU)"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.A."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Missouri--Columbia"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Access to files is limited to the campuses of the University of Missouri with SSO login."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10355/5811"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.","Title from title screen of research.pdf file viewed on (June 30, 2006)","Includes bibliographical references.","Vita.","Thesis (M.A.) University of Missouri-Columbia 2004.","Dissertations, Academic -- University of Missouri--Columbia -- Animal sciences."]},{"key":"dc:description.abstract","label":"Abstract","values":["[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] This study was performed to determine whether using high power microscopic analysis and two putative biochemical fertility markers, 15-lipoxygenase (15-LOX) and ubiquitin (UBI), could be used to accurately evaluate boar semen quality. In this study, ejaculates (n=116) were collected from 18 Large White boars for eight months (April, August-February). Ejaculates were analyzed and the percentage of proximal CDs (PCD), distal CDs (DCD), distal midpiece reflex CDs (DMR) and other spermatozoa abnormalities (ABN). Further ejaculate analysis was performed by semi-quantitative western blotting with computerized densitometry using antibodies against 15-LOX and UBI. Fertility data including FR and TNB was collected from sows mated to the boars in the study. Correlation coefficient analysis found moderate, negative relationships between FRs and DCDs (-0.39, P<0.01) and DMRs (-0.24, P<0.05), but not PCDs. These data suggest that an accurate boar semen analysis can be achieved by improved microscopy and by the use of biochemical fertility markers."]},{"key":"dc:title","label":"Title","values":["Arachidonate 15-lipoxygenase and ubiquitin as potential fertility markers in boars"]}]}],"canonical_facts":{"dc:contributor.advisor":["Safranski, Timothy Jon, 1965-","Sutovsky, Peter"],"dc:creator":["Lovercamp, Kyle W."],"dc:date.accessioned":["2010-02-23T19:52:39Z"],"dc:date.available":["2010-02-23T19:52:39Z"],"dc:date.issued":["2004"],"dc:description":["The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.","Title from title screen of research.pdf file viewed on (June 30, 2006)","Includes bibliographical references.","Vita.","Thesis (M.A.) University of Missouri-Columbia 2004.","Dissertations, Academic -- University of Missouri--Columbia -- Animal sciences."],"dc:description.abstract":["[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] This study was performed to determine whether using high power microscopic analysis and two putative biochemical fertility markers, 15-lipoxygenase (15-LOX) and ubiquitin (UBI), could be used to accurately evaluate boar semen quality. In this study, ejaculates (n=116) were collected from 18 Large White boars for eight months (April, August-February). Ejaculates were analyzed and the percentage of proximal CDs (PCD), distal CDs (DCD), distal midpiece reflex CDs (DMR) and other spermatozoa abnormalities (ABN). Further ejaculate analysis was performed by semi-quantitative western blotting with computerized densitometry using antibodies against 15-LOX and UBI. Fertility data including FR and TNB was collected from sows mated to the boars in the study. Correlation coefficient analysis found moderate, negative relationships between FRs and DCDs (-0.39, P<0.01) and DMRs (-0.24, P<0.05), but not PCDs. 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