{"id":{"repo_id":"missouri","oai_identifier":"oai:mospace.umsystem.edu:10355/5038"},"canonical_url":"https://search.dev.ndltd.org/etd/missouri/oai:mospace.umsystem.edu:10355/5038","repository":{"repo_id":"missouri","name":"University of Missouri","base_url":"https://mospace.umsystem.edu/oai/request"},"display":{"title":"The effects of histone acetylation on the maize allele PL1-blotched","abstract":"Covalent modifications of DNA and nucleosomal histone proteins associated with eukaryotic chromatin have the potential to alter expression of a gene without change in its DNA sequence. One gene regulated through this so-called epigenetic process is the Pl1- Blotched gene of maize. Pl1-Blotched is a allele of the purple1 (pl1) gene, which encodes a transcription factor that activates synthesis of purple anthocyanin pigments. Pl1-Blotched is unusual in that it leads to variegated, rather than uniform, pigmentation. At the molecular level, this phenotype is associated with low expression of pl1 mRNA, a novel pattern of DNA methylation and condensed chromatin structure. To ask if acetylation of nucleosomal histone proteins might be involved in regulating the chromatin organization of Pl1-Blotched, this allele was crossed into 40 lines containing transgenes that target reduction of various histone acetyltransferase and histone deacetylase genes. Some of the lines led to altered Pl1-Blotched pigmentation. Detailed analysis of molecular changes underlying the altered pigmentation resulted in models that implicate both histone acetyltransferase and histone deacetylase genes in controlling expression of Pl1-Blotched. These findings lay the foundation for future studies aimed at further understanding the interplay between histone modification and regulation of gene expression.","abstract_html":"Covalent modifications of DNA and nucleosomal histone proteins associated with eukaryotic chromatin have the potential to alter expression of a gene without change in its DNA sequence. One gene regulated through this so-called epigenetic process is the Pl1- Blotched gene of maize. Pl1-Blotched is a allele of the purple1 (pl1) gene, which encodes a transcription factor that activates synthesis of purple anthocyanin pigments. Pl1-Blotched is unusual in that it leads to variegated, rather than uniform, pigmentation. At the molecular level, this phenotype is associated with low expression of pl1 mRNA, a novel pattern of DNA methylation and condensed chromatin structure. To ask if acetylation of nucleosomal histone proteins might be involved in regulating the chromatin organization of Pl1-Blotched, this allele was crossed into 40 lines containing transgenes that target reduction of various histone acetyltransferase and histone deacetylase genes. Some of the lines led to altered Pl1-Blotched pigmentation. Detailed analysis of molecular changes underlying the altered pigmentation resulted in models that implicate both histone acetyltransferase and histone deacetylase genes in controlling expression of Pl1-Blotched. These findings lay the foundation for future studies aimed at further understanding the interplay between histone modification and regulation of gene expression.","abstract_has_math":false,"creators":["Ladipo, Paul B."],"institution":"University of Missouri--Columbia","degree_name":"M.A.","degree_level":"Masters","degree_discipline":"Biological sciences (MU)","degree_department":null,"school":null,"contributors":[],"advisors":["Cone, Karen Camille, 1952-"],"committee_chairs":[],"committee_members":[],"year":2007,"date_issued":"2007","date_published":"2007","updated_at":"2026-07-24T03:07:13Z","subjects":[],"languages":["eng","English"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.doi","label":"DOI","values":["https://doi.org/10.32469/10355/5038"],"render_values":[{"text":"https://doi.org/10.32469/10355/5038","href":"https://doi.org/10.32469/10355/5038","code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/10355/5038","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Cone, Karen Camille, 1952-"]},{"key":"dc:creator","label":"Author","values":["Ladipo, Paul B."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2010-01-12T19:08:51Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2010-01-12T19:08:51Z"]},{"key":"dc:date.issued","label":"Date","values":["2007"]},{"key":"dc:publisher","label":"Institution","values":["University of Missouri--Columbia"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biological sciences (MU)"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.A."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Missouri--Columbia"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.doi","label":"DOI","values":["https://doi.org/10.32469/10355/5038"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10355/5038"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.","Title from title screen of research.pdf file (viewed on September 29, 2008)","Includes bibliographical references.","Thesis (M.A.) University of Missouri-Columbia 2007.","Dissertations, Academic -- University of Missouri--Columbia -- Biological sciences."]},{"key":"dc:description.abstract","label":"Abstract","values":["Covalent modifications of DNA and nucleosomal histone proteins associated with eukaryotic chromatin have the potential to alter expression of a gene without change in its DNA sequence. One gene regulated through this so-called epigenetic process is the Pl1- Blotched gene of maize. Pl1-Blotched is a allele of the purple1 (pl1) gene, which encodes a transcription factor that activates synthesis of purple anthocyanin pigments. Pl1-Blotched is unusual in that it leads to variegated, rather than uniform, pigmentation. At the molecular level, this phenotype is associated with low expression of pl1 mRNA, a novel pattern of DNA methylation and condensed chromatin structure. To ask if acetylation of nucleosomal histone proteins might be involved in regulating the chromatin organization of Pl1-Blotched, this allele was crossed into 40 lines containing transgenes that target reduction of various histone acetyltransferase and histone deacetylase genes. Some of the lines led to altered Pl1-Blotched pigmentation. Detailed analysis of molecular changes underlying the altered pigmentation resulted in models that implicate both histone acetyltransferase and histone deacetylase genes in controlling expression of Pl1-Blotched. These findings lay the foundation for future studies aimed at further understanding the interplay between histone modification and regulation of gene expression."]},{"key":"dc:source","label":"Dc Source","values":["Submitted by University of Missouri--Columbia Graduate School."]},{"key":"dc:title","label":"Title","values":["The effects of histone acetylation on the maize allele PL1-blotched"]}]}],"canonical_facts":{"dc:contributor.advisor":["Cone, Karen Camille, 1952-"],"dc:creator":["Ladipo, Paul B."],"dc:date.accessioned":["2010-01-12T19:08:51Z"],"dc:date.available":["2010-01-12T19:08:51Z"],"dc:date.issued":["2007"],"dc:description":["The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.","Title from title screen of research.pdf file (viewed on September 29, 2008)","Includes bibliographical references.","Thesis (M.A.) University of Missouri-Columbia 2007.","Dissertations, Academic -- University of Missouri--Columbia -- Biological sciences."],"dc:description.abstract":["Covalent modifications of DNA and nucleosomal histone proteins associated with eukaryotic chromatin have the potential to alter expression of a gene without change in its DNA sequence. One gene regulated through this so-called epigenetic process is the Pl1- Blotched gene of maize. Pl1-Blotched is a allele of the purple1 (pl1) gene, which encodes a transcription factor that activates synthesis of purple anthocyanin pigments. Pl1-Blotched is unusual in that it leads to variegated, rather than uniform, pigmentation. At the molecular level, this phenotype is associated with low expression of pl1 mRNA, a novel pattern of DNA methylation and condensed chromatin structure. To ask if acetylation of nucleosomal histone proteins might be involved in regulating the chromatin organization of Pl1-Blotched, this allele was crossed into 40 lines containing transgenes that target reduction of various histone acetyltransferase and histone deacetylase genes. Some of the lines led to altered Pl1-Blotched pigmentation. Detailed analysis of molecular changes underlying the altered pigmentation resulted in models that implicate both histone acetyltransferase and histone deacetylase genes in controlling expression of Pl1-Blotched. These findings lay the foundation for future studies aimed at further understanding the interplay between histone modification and regulation of gene expression."],"dc:identifier.doi":["https://doi.org/10.32469/10355/5038"],"dc:identifier.uri":["https://hdl.handle.net/10355/5038"],"dc:language":["English"],"dc:language.iso":["eng"],"dc:publisher":["University of Missouri--Columbia"],"dc:source":["Submitted by University of Missouri--Columbia Graduate School."],"dc:title":["The effects of histone acetylation on the maize allele PL1-blotched"],"dc:type":["Thesis"],"thesis:degree_discipline":["Biological sciences (MU)"],"thesis:degree_level":["Masters"],"thesis:degree_name":["M.A."],"thesis:institution_name":["University of Missouri--Columbia"]},"updated_at":"2026-07-24T03:07:13Z"}