{"id":{"repo_id":"missouri","oai_identifier":"oai:mospace.umsystem.edu:10355/49027"},"canonical_url":"https://search.dev.ndltd.org/etd/missouri/oai:mospace.umsystem.edu:10355/49027","repository":{"repo_id":"missouri","name":"University of Missouri","base_url":"https://mospace.umsystem.edu/oai/request"},"display":{"title":"Posttranscriptional gene regulation of CD4+ T cell cytokine expression by the RNA binding protein HuR","abstract":"[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI-COLUMBIA AT AUTHOR'S REQUEST.] Posttranscriptional gene regulation by RNA-binding proteins such as HuR fine tunes gene expression in T cells leading to powerful effects on immune responses. HuR can stabilize target mRNAs and/or promote translation by interacting with their 3-UTR AUrich elements. We discovered that the interaction of HuR with the Il2ra (CD25) mRNA transcript is required for its optimal translation. Conditional HuR knockout (KO) in CD4+ T cells resulted in loss of IL-2 homeostasis and defects in Th2 cytokine production. In an allergic airway inflammatory model, OVA-challenged HuR KO mice had decreased lung cellular infiltration, eosinophilia and IL-13. These results demonstrate that HuR plays a pivotal role in maintaining normal IL-2 homeostasis, augmenting Th2 differentiation and promoting allergic airway inflammation.","abstract_html":"[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI-COLUMBIA AT AUTHOR'S REQUEST.] Posttranscriptional gene regulation by RNA-binding proteins such as HuR fine tunes gene expression in T cells leading to powerful effects on immune responses. HuR can stabilize target mRNAs and/or promote translation by interacting with their 3-UTR AUrich elements. We discovered that the interaction of HuR with the Il2ra (CD25) mRNA transcript is required for its optimal translation. Conditional HuR knockout (KO) in CD4+ T cells resulted in loss of IL-2 homeostasis and defects in Th2 cytokine production. In an allergic airway inflammatory model, OVA-challenged HuR KO mice had decreased lung cellular infiltration, eosinophilia and IL-13. These results demonstrate that HuR plays a pivotal role in maintaining normal IL-2 homeostasis, augmenting Th2 differentiation and promoting allergic airway inflammation.","abstract_has_math":false,"creators":["Techasintana, Patsharaporn"],"institution":"University of Missouri--Columbia","degree_name":"Ph. D.","degree_level":"Doctoral","degree_discipline":"Microbiology (Medicine) (MU)","degree_department":null,"school":null,"contributors":[],"advisors":["Atasoy, Ulus"],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015","date_published":"2015","updated_at":"2026-07-24T03:08:32Z","subjects":[],"languages":["eng","English"],"rights":["Access to files is limited to the University of Missouri--Columbia with SSO login."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.doi","label":"DOI","values":["https://doi.org/10.32469/10355/49027"],"render_values":[{"text":"https://doi.org/10.32469/10355/49027","href":"https://doi.org/10.32469/10355/49027","code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/10355/49027","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Atasoy, Ulus"]},{"key":"dc:creator","label":"Author","values":["Techasintana, Patsharaporn"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2016-05-05T18:13:02Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2016-05-05T18:13:02Z"]},{"key":"dc:date.issued","label":"Date","values":["2015"]},{"key":"dc:publisher","label":"Institution","values":["University of Missouri--Columbia"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology (Medicine) (MU)"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph. 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HuR can stabilize target mRNAs and/or promote translation by interacting with their 3-UTR AUrich elements. We discovered that the interaction of HuR with the Il2ra (CD25) mRNA transcript is required for its optimal translation. Conditional HuR knockout (KO) in CD4+ T cells resulted in loss of IL-2 homeostasis and defects in Th2 cytokine production. In an allergic airway inflammatory model, OVA-challenged HuR KO mice had decreased lung cellular infiltration, eosinophilia and IL-13. These results demonstrate that HuR plays a pivotal role in maintaining normal IL-2 homeostasis, augmenting Th2 differentiation and promoting allergic airway inflammation."]},{"key":"dc:title","label":"Title","values":["Posttranscriptional gene regulation of CD4+ T cell cytokine expression by the RNA binding protein HuR"]}]}],"canonical_facts":{"dc:contributor.advisor":["Atasoy, Ulus"],"dc:creator":["Techasintana, Patsharaporn"],"dc:date.accessioned":["2016-05-05T18:13:02Z"],"dc:date.available":["2016-05-05T18:13:02Z"],"dc:date.issued":["2015"],"dc:description.abstract":["[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI-COLUMBIA AT AUTHOR'S REQUEST.] Posttranscriptional gene regulation by RNA-binding proteins such as HuR fine tunes gene expression in T cells leading to powerful effects on immune responses. HuR can stabilize target mRNAs and/or promote translation by interacting with their 3-UTR AUrich elements. We discovered that the interaction of HuR with the Il2ra (CD25) mRNA transcript is required for its optimal translation. Conditional HuR knockout (KO) in CD4+ T cells resulted in loss of IL-2 homeostasis and defects in Th2 cytokine production. In an allergic airway inflammatory model, OVA-challenged HuR KO mice had decreased lung cellular infiltration, eosinophilia and IL-13. These results demonstrate that HuR plays a pivotal role in maintaining normal IL-2 homeostasis, augmenting Th2 differentiation and promoting allergic airway inflammation."],"dc:identifier.doi":["https://doi.org/10.32469/10355/49027"],"dc:identifier.uri":["https://hdl.handle.net/10355/49027"],"dc:language":["English"],"dc:language.iso":["eng"],"dc:publisher":["University of Missouri--Columbia"],"dc:rights":["Access to files is limited to the University of Missouri--Columbia with SSO login."],"dc:title":["Posttranscriptional gene regulation of CD4+ T cell cytokine expression by the RNA binding protein HuR"],"dc:type":["Thesis"],"thesis:degree_discipline":["Microbiology (Medicine) (MU)"],"thesis:degree_level":["Doctoral"],"thesis:degree_name":["Ph. D."],"thesis:institution_name":["University of Missouri--Columbia"]},"updated_at":"2026-07-24T03:08:32Z"}