{"id":{"repo_id":"missouri","oai_identifier":"oai:mospace.umsystem.edu:10355/40209"},"canonical_url":"https://search.dev.ndltd.org/etd/missouri/oai:mospace.umsystem.edu:10355/40209","repository":{"repo_id":"missouri","name":"University of Missouri","base_url":"https://mospace.umsystem.edu/oai/request"},"display":{"title":"Co-ordinated expression of CRTB, At-VTE3, and VTE4 to enhance pro-vitamin A and vitamin E in transgenic soybean","abstract":"Although soybean is an excellent general nutritional source, it is not very rich in particular vitamins. The main goal of this study is to enhance both pro-vitamin A (carotenoids) and vitamin E (tocopherols) content in soybean seeds. We have genetically engineered the carotenoid and the tocopherol biosynthetic pathways in soybean seeds by ectopically expressing three genes: Erwinia uredovora phytoene synthase (crtB) to increase carotenoid content, Arabidopsis 2-methyl-6-phytylbenzoquinol methyl transferase (At-VTE3) and soybean [gamma]-tocopherol methyl transpherase (VTE4) to increase [alpha]-tocopherol using the self-cleavage activity of FMDV 2A sequence to join two adjacent proteins. This 2A-polyprotein construct was introduced to soybean via Agrobacteriummediated cotyledonary node transformation method. One inheritable transgenic event displayed \"golden\"-colored seeds. The presence and expression of the three genes were detected in golden soybean event HYX-7-1 by qRT-PCR analyses. HPLC analysis of individual golden soybean lines revealed that the seeds accumulated as high as 128[mu]g/g of total carotenoids, approximately 25- fold higher than wild type and 45-fold higher than empty vector control seeds. Of total carotenoids, 98% was pro-vitamin A ([beta]-carotene and [beta]-carotene equivalents). By contrast, golden seeds showed a decrease of tochopherol and a significant change of fatty acid profile. Transgenic golden soybean lines also displayed a delay in germination with a decreased rate (50%) and dwarf phenotype at early developmental stage, but showed normal development later.","abstract_html":"Although soybean is an excellent general nutritional source, it is not very rich in particular vitamins. The main goal of this study is to enhance both pro-vitamin A (carotenoids) and vitamin E (tocopherols) content in soybean seeds. We have genetically engineered the carotenoid and the tocopherol biosynthetic pathways in soybean seeds by ectopically expressing three genes: Erwinia uredovora phytoene synthase (crtB) to increase carotenoid content, Arabidopsis 2-methyl-6-phytylbenzoquinol methyl transferase (At-VTE3) and soybean [gamma]-tocopherol methyl transpherase (VTE4) to increase [alpha]-tocopherol using the self-cleavage activity of FMDV 2A sequence to join two adjacent proteins. This 2A-polyprotein construct was introduced to soybean via Agrobacteriummediated cotyledonary node transformation method. One inheritable transgenic event displayed "golden"-colored seeds. The presence and expression of the three genes were detected in golden soybean event HYX-7-1 by qRT-PCR analyses. HPLC analysis of individual golden soybean lines revealed that the seeds accumulated as high as 128[mu]g/g of total carotenoids, approximately 25- fold higher than wild type and 45-fold higher than empty vector control seeds. Of total carotenoids, 98% was pro-vitamin A ([beta]-carotene and [beta]-carotene equivalents). By contrast, golden seeds showed a decrease of tochopherol and a significant change of fatty acid profile. Transgenic golden soybean lines also displayed a delay in germination with a decreased rate (50%) and dwarf phenotype at early developmental stage, but showed normal development later.","abstract_has_math":false,"creators":["Pham, Dung Thu"],"institution":"University of Missouri--Columbia","degree_name":"M.S.","degree_level":"Masters","degree_discipline":"Plant sciences (MU)","degree_department":null,"school":null,"contributors":[],"advisors":["Zhang, Zhanyuan"],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013","date_published":"2013","updated_at":"2026-07-24T03:09:27Z","subjects":[],"languages":["eng","English"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://doi.org/10.32469/10355/40209"],"render_values":[{"text":"https://doi.org/10.32469/10355/40209","href":"https://doi.org/10.32469/10355/40209","code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/10355/40209","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Zhang, Zhanyuan"]},{"key":"dc:creator","label":"Author","values":["Pham, Dung Thu"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2013-11-20T15:06:00Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2013-11-20T15:06:00Z"]},{"key":"dc:date.issued","label":"Date","values":["2013"]},{"key":"dc:publisher","label":"Institution","values":["University of Missouri--Columbia"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Plant sciences (MU)"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Missouri--Columbia"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10355/40209","https://doi.org/10.32469/10355/40209"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["July 2013.","A Thesis presented to the Faculty of the Graduate School at the University of Missouri--Columbia In Partial Fulfillment of the Requirements for the Degree Master of Science.","Thesis supervisor: Dr. Zhanyuan J Zhang."]},{"key":"dc:description.abstract","label":"Abstract","values":["Although soybean is an excellent general nutritional source, it is not very rich in particular vitamins. The main goal of this study is to enhance both pro-vitamin A (carotenoids) and vitamin E (tocopherols) content in soybean seeds. We have genetically engineered the carotenoid and the tocopherol biosynthetic pathways in soybean seeds by ectopically expressing three genes: Erwinia uredovora phytoene synthase (crtB) to increase carotenoid content, Arabidopsis 2-methyl-6-phytylbenzoquinol methyl transferase (At-VTE3) and soybean [gamma]-tocopherol methyl transpherase (VTE4) to increase [alpha]-tocopherol using the self-cleavage activity of FMDV 2A sequence to join two adjacent proteins. This 2A-polyprotein construct was introduced to soybean via Agrobacteriummediated cotyledonary node transformation method. One inheritable transgenic event displayed \"golden\"-colored seeds. The presence and expression of the three genes were detected in golden soybean event HYX-7-1 by qRT-PCR analyses. HPLC analysis of individual golden soybean lines revealed that the seeds accumulated as high as 128[mu]g/g of total carotenoids, approximately 25- fold higher than wild type and 45-fold higher than empty vector control seeds. Of total carotenoids, 98% was pro-vitamin A ([beta]-carotene and [beta]-carotene equivalents). By contrast, golden seeds showed a decrease of tochopherol and a significant change of fatty acid profile. Transgenic golden soybean lines also displayed a delay in germination with a decreased rate (50%) and dwarf phenotype at early developmental stage, but showed normal development later."]},{"key":"dc:source","label":"Dc Source","values":["Submitted by the University of Missouri--Columbia Graduate School"]},{"key":"dc:title","label":"Title","values":["Co-ordinated expression of CRTB, At-VTE3, and VTE4 to enhance pro-vitamin A and vitamin E in transgenic soybean"]}]}],"canonical_facts":{"dc:contributor.advisor":["Zhang, Zhanyuan"],"dc:creator":["Pham, Dung Thu"],"dc:date.accessioned":["2013-11-20T15:06:00Z"],"dc:date.available":["2013-11-20T15:06:00Z"],"dc:date.issued":["2013"],"dc:description":["July 2013.","A Thesis presented to the Faculty of the Graduate School at the University of Missouri--Columbia In Partial Fulfillment of the Requirements for the Degree Master of Science.","Thesis supervisor: Dr. Zhanyuan J Zhang."],"dc:description.abstract":["Although soybean is an excellent general nutritional source, it is not very rich in particular vitamins. The main goal of this study is to enhance both pro-vitamin A (carotenoids) and vitamin E (tocopherols) content in soybean seeds. We have genetically engineered the carotenoid and the tocopherol biosynthetic pathways in soybean seeds by ectopically expressing three genes: Erwinia uredovora phytoene synthase (crtB) to increase carotenoid content, Arabidopsis 2-methyl-6-phytylbenzoquinol methyl transferase (At-VTE3) and soybean [gamma]-tocopherol methyl transpherase (VTE4) to increase [alpha]-tocopherol using the self-cleavage activity of FMDV 2A sequence to join two adjacent proteins. This 2A-polyprotein construct was introduced to soybean via Agrobacteriummediated cotyledonary node transformation method. One inheritable transgenic event displayed \"golden\"-colored seeds. The presence and expression of the three genes were detected in golden soybean event HYX-7-1 by qRT-PCR analyses. HPLC analysis of individual golden soybean lines revealed that the seeds accumulated as high as 128[mu]g/g of total carotenoids, approximately 25- fold higher than wild type and 45-fold higher than empty vector control seeds. Of total carotenoids, 98% was pro-vitamin A ([beta]-carotene and [beta]-carotene equivalents). By contrast, golden seeds showed a decrease of tochopherol and a significant change of fatty acid profile. Transgenic golden soybean lines also displayed a delay in germination with a decreased rate (50%) and dwarf phenotype at early developmental stage, but showed normal development later."],"dc:identifier.uri":["https://hdl.handle.net/10355/40209","https://doi.org/10.32469/10355/40209"],"dc:language":["English"],"dc:language.iso":["eng"],"dc:publisher":["University of Missouri--Columbia"],"dc:source":["Submitted by the University of Missouri--Columbia Graduate School"],"dc:title":["Co-ordinated expression of CRTB, At-VTE3, and VTE4 to enhance pro-vitamin A and vitamin E in transgenic soybean"],"dc:type":["Thesis"],"thesis:degree_discipline":["Plant sciences (MU)"],"thesis:degree_level":["Masters"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Missouri--Columbia"]},"updated_at":"2026-07-24T03:09:27Z"}