The feasibility of interspecific rescue of endothelial/hematopoietic lineage deficiency

Here we propose to develop a mouse model which will establish “proof of principle” for future development of large animal (e.g. pig) models which could be used as actual human solid organ and blood donors as well as in pre-clinic trials. As a proof of concept, we intended to develop interspecific chimeras between mouse and rat to test the possibility that embryonic stem cells (ESCs) from one species are able to survive and develop in a blastocyst from a difference species. Then we intended to make a rat-mouse chimera with an endothelium/hematopoietic system from a rat genetic background and the other tissues and organs from a mouse genetic background. The first series of experiments of this thesis work were designed to test the feasibility for the interspecific chimeras (mouse-rat and rat-mouse) that can be made by ESC-blastocyst injection method. The second series of experiments of this thesis work were designed to test the feasibility of making such a chimera by using rat ESCs to rescue endothelial/hematopoietic system deficient mouse embryos that will otherwise die.

Name thesis:degree_name

Ph. D.

Level thesis:degree_level

Doctoral

Discipline thesis:degree_discipline

Veterinary pathobiology (MU)

Grantor dc:publisher

University of Missouri--Columbia

Year dc:date.issued

2012

Author dc:creator

• Zhao, Chongbei

Advisors dc:contributor.advisor

• Critser, John Kenneth
• Wells, Kevin D.
• Bryda, Elizabeth C.

• embryonic stem cells
• interspecific chimeras
• organ donation
• blood donation

Statement dc:rights

• OpenAccess.

Language dc:language.iso

eng, English

DOI

https://doi.org/10.32469/10355/15129

OAI identifier oai:identifier

oai:mospace.umsystem.edu:10355/15129