Investigating the role of germline RNA helicase-1 in Caenorhabditis elegans
type: CreativeWork
description: [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] The germline specific RNA helicase GLH-1, a constitutive P-granule component, and DCR-1, the riboendonuclease responsible for processing small RNAs in the miRNA pathway, have been shown to be interdependent in the C. elegans germline. The work described here is based on the hypothesis that if GLH-1 functions with DCR-1 in the miRNA pathway in the C. elegans germline, then when GLH-1 is missing or reduced, there will be a difference in miRNA processing and/or abundance in glh-1 mutants as compared to wild type worms. We utilized deep sequencing on an Illumina platform to analyze differences in miRNA abundance between glh-1(gk100) mutant and wild type worms. In addition, to ascertain whether any miRNAs are germline specific, we also analyzed differences in miRNA expression between glp-4(bn2) worms cultured at both 15oC and 26oC. Results of deep sequencing and qRT-PCR indicated that in general miRNA abundance is moderately decreased in glh-1(gk100) mutants, and this reduction is significant in mutants cultured at 26oC. Our results also indicated that 11 of the 153 miRNAs identified to date in C. elegans are germline-enhanced, including miR-38, miR-40, miR-228, and miR-244, and may be germline-specific.
abstract: [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] The germline specific RNA helicase GLH-1, a constitutive P-granule component, and DCR-1, the riboendonuclease responsible for processing small RNAs in the miRNA pathway, have been shown to be interdependent in the C. elegans germline. The work described here is based on the hypothesis that if GLH-1 functions with DCR-1 in the miRNA pathway in the C. elegans germline, then when GLH-1 is missing or reduced, there will be a difference in miRNA processing and/or abundance in glh-1 mutants as compared to wild type worms. We utilized deep sequencing on an Illumina platform to analyze differences in miRNA abundance between glh-1(gk100) mutant and wild type worms. In addition, to ascertain whether any miRNAs are germline specific, we also analyzed differences in miRNA expression between glp-4(bn2) worms cultured at both 15oC and 26oC. Results of deep sequencing and qRT-PCR indicated that in general miRNA abundance is moderately decreased in glh-1(gk100) mutants, and this reduction is significant in mutants cultured at 26oC. Our results also indicated that 11 of the 153 miRNAs identified to date in C. elegans are germline-enhanced, including miR-38, miR-40, miR-228, and miR-244, and may be germline-specific.
author: McEwen, Tamara J., 1965-
language: eng
language: English
keywords: germline development, miRNA pathway, C. elegans
date published: 2011
date modified: 2026-07-24T03:09:05Z
provider: University of Missouri
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Missouri--Columbia

PropertyValue
property ID: Handle
value: https://hdl.handle.net/10355/14294

PropertyValue
property ID: DOI
value: 10.32469/10355/14294