{"id":{"repo_id":"missouri","oai_identifier":"oai:mospace.umsystem.edu:10355/10129"},"canonical_url":"https://search.dev.ndltd.org/etd/missouri/oai:mospace.umsystem.edu:10355/10129","repository":{"repo_id":"missouri","name":"University of Missouri","base_url":"https://mospace.umsystem.edu/oai/request"},"display":{"title":"High throughput profiling of transcription factors involved in soybean root growth under water deficit","abstract":"Drought is the major abiotic stress factor limiting crop productivity worldwide. Plant root and shoot systems respond to environmental changes by altering the expression of complex gene networks through sensing environmental stresses and modifying signaling and metabolic pathways. Previous work (Yamaguchi et al., 2009) showed that the soybean primary root adapts to low water potential (-1.6 MPa) by maintaining longitudinal expansion in the apical 4 mm (region 1), whereas in the adjacent 4 mm (region 2), longitudinal expansion reaches a maximum in well-watered roots but is progressively inhibited at low water potential. To identify the key transcription factors (TFs) that determine these responses to low water potential, we have conducted high-throughput profiling of root-related TF expression in regions 1 and 2 of water-stressed and well-watered roots using quantitative real-time PCR. 186 root- and stress-related TFs were selected to identify their specific expression patterns in root regions 1 and 2 of well-watered and water-stressed soybean seedlings at four time points (5h, 12h, 24h, and 48h) after transplanting. Several stress-specific and root-region-specific transcripts were identified which may contribute to root responses to water deficits. Among these were zinc-finger protein, MYB-related protein, GmNAC3, GmNAC4, and bZIP transcription factors. These TFs were differentially expressed in distinct root regions, and therefore they can be targeted for functional characterization and further genetic engineering for enhanced drought resistance in soybean.","abstract_html":"Drought is the major abiotic stress factor limiting crop productivity worldwide. Plant root and shoot systems respond to environmental changes by altering the expression of complex gene networks through sensing environmental stresses and modifying signaling and metabolic pathways. Previous work (Yamaguchi et al., 2009) showed that the soybean primary root adapts to low water potential (-1.6 MPa) by maintaining longitudinal expansion in the apical 4 mm (region 1), whereas in the adjacent 4 mm (region 2), longitudinal expansion reaches a maximum in well-watered roots but is progressively inhibited at low water potential. To identify the key transcription factors (TFs) that determine these responses to low water potential, we have conducted high-throughput profiling of root-related TF expression in regions 1 and 2 of water-stressed and well-watered roots using quantitative real-time PCR. 186 root- and stress-related TFs were selected to identify their specific expression patterns in root regions 1 and 2 of well-watered and water-stressed soybean seedlings at four time points (5h, 12h, 24h, and 48h) after transplanting. Several stress-specific and root-region-specific transcripts were identified which may contribute to root responses to water deficits. Among these were zinc-finger protein, MYB-related protein, GmNAC3, GmNAC4, and bZIP transcription factors. These TFs were differentially expressed in distinct root regions, and therefore they can be targeted for functional characterization and further genetic engineering for enhanced drought resistance in soybean.","abstract_has_math":false,"creators":["Tran, Huong Nguyen Thanh, 1983-"],"institution":"University of Missouri--Columbia","degree_name":"M.S.","degree_level":"Masters","degree_discipline":"Plant sciences (MU)","degree_department":null,"school":null,"contributors":[],"advisors":["Nguyen, Henry T."],"committee_chairs":[],"committee_members":[],"year":2009,"date_issued":"2009","date_published":"2009","updated_at":"2026-07-24T03:08:32Z","subjects":[],"languages":["eng","English"],"rights":["OpenAccess."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.doi","label":"DOI","values":["https://doi.org/10.32469/10355/10129"],"render_values":[{"text":"https://doi.org/10.32469/10355/10129","href":"https://doi.org/10.32469/10355/10129","code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/10355/10129","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Nguyen, Henry T."]},{"key":"dc:creator","label":"Author","values":["Tran, Huong Nguyen Thanh, 1983-"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2011-03-04T15:11:52Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2011-03-04T15:11:52Z"]},{"key":"dc:date.issued","label":"Date","values":["2009"]},{"key":"dc:publisher","label":"Institution","values":["University of Missouri--Columbia"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Plant sciences (MU)"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Missouri--Columbia"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["OpenAccess."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.doi","label":"DOI","values":["https://doi.org/10.32469/10355/10129"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10355/10129"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Title from PDF of title page (University of Missouri--Columbia, viewed on February 22, 2011).","The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.","Thesis advisor: Dr. Henry T. 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To identify the key transcription factors (TFs) that determine these responses to low water potential, we have conducted high-throughput profiling of root-related TF expression in regions 1 and 2 of water-stressed and well-watered roots using quantitative real-time PCR. 186 root- and stress-related TFs were selected to identify their specific expression patterns in root regions 1 and 2 of well-watered and water-stressed soybean seedlings at four time points (5h, 12h, 24h, and 48h) after transplanting. Several stress-specific and root-region-specific transcripts were identified which may contribute to root responses to water deficits. Among these were zinc-finger protein, MYB-related protein, GmNAC3, GmNAC4, and bZIP transcription factors. These TFs were differentially expressed in distinct root regions, and therefore they can be targeted for functional characterization and further genetic engineering for enhanced drought resistance in soybean."]},{"key":"dc:title","label":"Title","values":["High throughput profiling of transcription factors involved in soybean root growth under water deficit"]}]}],"canonical_facts":{"dc:contributor.advisor":["Nguyen, Henry T."],"dc:creator":["Tran, Huong Nguyen Thanh, 1983-"],"dc:date.accessioned":["2011-03-04T15:11:52Z"],"dc:date.available":["2011-03-04T15:11:52Z"],"dc:date.issued":["2009"],"dc:description":["Title from PDF of title page (University of Missouri--Columbia, viewed on February 22, 2011).","The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.","Thesis advisor: Dr. Henry T. Nguyen.","M. S. 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To identify the key transcription factors (TFs) that determine these responses to low water potential, we have conducted high-throughput profiling of root-related TF expression in regions 1 and 2 of water-stressed and well-watered roots using quantitative real-time PCR. 186 root- and stress-related TFs were selected to identify their specific expression patterns in root regions 1 and 2 of well-watered and water-stressed soybean seedlings at four time points (5h, 12h, 24h, and 48h) after transplanting. Several stress-specific and root-region-specific transcripts were identified which may contribute to root responses to water deficits. Among these were zinc-finger protein, MYB-related protein, GmNAC3, GmNAC4, and bZIP transcription factors. 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