{"id":{"repo_id":"mississippi","oai_identifier":"oai:egrove.olemiss.edu:etd-2354"},"canonical_url":"https://search.dev.ndltd.org/etd/mississippi/oai:egrove.olemiss.edu:etd-2354","repository":{"repo_id":"mississippi","name":"University of Mississippi","base_url":"https://egrove.olemiss.edu/do/oai/"},"display":{"title":"Autoregulation of the Glial Gene reversed polarity in Drosophila melanogaster","abstract":"During development, cells of the nervous system begin as unspecified precursors and proceed along one of two developmental paths to become either neurons or glia. I seek to understand more about the genes that control this process, focusing on the lesser understood of the cell types, glial cells. Using Drosophila melanogaster as a model system, previous work from my lab and others has established the role of the master regulatory transcription factor Gcm in directing neuronal precursor cells to assume a lateral glial fate. Gcm acts on many target genes, one of which is reversed polarity (repo). repo is necessary for proper glial cell differentiation; once activated, its expression is maintained throughout the life of the fly through currently unknown mechanisms. I propose that repo expression is maintained in an autoregulatory manner, whereby Repo protein acts as a transcription factor on its own regulatory DNA sequence. Three canonical Repo binding sites (RBSs) are located within the 4.3 kb repo cis-regulatory DNA (CRD). Using both S2 cell culture and in vivo expression systems, I have evidence that suggests Repo protein interacts strongly with one of these sites, designated RBS1, to induce the expression of reporter genes. Mutagenesis of RBS1 results in a significant decrease of reporter gene expression in both systems, while RBS2 and RBS3 appear to have no role in autoregulation of repo expression.","abstract_html":"During development, cells of the nervous system begin as unspecified precursors and proceed along one of two developmental paths to become either neurons or glia. I seek to understand more about the genes that control this process, focusing on the lesser understood of the cell types, glial cells. Using Drosophila melanogaster as a model system, previous work from my lab and others has established the role of the master regulatory transcription factor Gcm in directing neuronal precursor cells to assume a lateral glial fate. Gcm acts on many target genes, one of which is reversed polarity (repo). repo is necessary for proper glial cell differentiation; once activated, its expression is maintained throughout the life of the fly through currently unknown mechanisms. I propose that repo expression is maintained in an autoregulatory manner, whereby Repo protein acts as a transcription factor on its own regulatory DNA sequence. Three canonical Repo binding sites (RBSs) are located within the 4.3 kb repo cis-regulatory DNA (CRD). Using both S2 cell culture and in vivo expression systems, I have evidence that suggests Repo protein interacts strongly with one of these sites, designated RBS1, to induce the expression of reporter genes. Mutagenesis of RBS1 results in a significant decrease of reporter gene expression in both systems, while RBS2 and RBS3 appear to have no role in autoregulation of repo expression.","abstract_has_math":false,"creators":["Wood, Jamie L."],"institution":null,"degree_name":"Ph.D. in Biological Science","degree_level":"Dissertation","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Bradley Jones","Michael Mossing","Brice P. Noonan"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-01-01T08:00:00Z","date_published":"2015-01-01T08:00:00Z","updated_at":"2026-07-24T03:06:44Z","subjects":["autoregulation","Drosophila","gcm","glia","repo","Biology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://egrove.olemiss.edu/etd/1355","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Bradley Jones","Michael Mossing","Brice P. 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I seek to understand more about the genes that control this process, focusing on the lesser understood of the cell types, glial cells. Using Drosophila melanogaster as a model system, previous work from my lab and others has established the role of the master regulatory transcription factor Gcm in directing neuronal precursor cells to assume a lateral glial fate. Gcm acts on many target genes, one of which is reversed polarity (repo). repo is necessary for proper glial cell differentiation; once activated, its expression is maintained throughout the life of the fly through currently unknown mechanisms. I propose that repo expression is maintained in an autoregulatory manner, whereby Repo protein acts as a transcription factor on its own regulatory DNA sequence. Three canonical Repo binding sites (RBSs) are located within the 4.3 kb repo cis-regulatory DNA (CRD). Using both S2 cell culture and in vivo expression systems, I have evidence that suggests Repo protein interacts strongly with one of these sites, designated RBS1, to induce the expression of reporter genes. Mutagenesis of RBS1 results in a significant decrease of reporter gene expression in both systems, while RBS2 and RBS3 appear to have no role in autoregulation of repo expression."]},{"key":"dc:title","label":"Title","values":["Autoregulation of the Glial Gene reversed polarity in Drosophila melanogaster"]}]}],"canonical_facts":{"dc:contributor":["Bradley Jones","Michael Mossing","Brice P. Noonan"],"dc:creator":["Wood, Jamie L."],"dc:date.available":["2020-01-23T08:00:00Z"],"dc:description.abstract":["During development, cells of the nervous system begin as unspecified precursors and proceed along one of two developmental paths to become either neurons or glia. I seek to understand more about the genes that control this process, focusing on the lesser understood of the cell types, glial cells. Using Drosophila melanogaster as a model system, previous work from my lab and others has established the role of the master regulatory transcription factor Gcm in directing neuronal precursor cells to assume a lateral glial fate. Gcm acts on many target genes, one of which is reversed polarity (repo). repo is necessary for proper glial cell differentiation; once activated, its expression is maintained throughout the life of the fly through currently unknown mechanisms. I propose that repo expression is maintained in an autoregulatory manner, whereby Repo protein acts as a transcription factor on its own regulatory DNA sequence. Three canonical Repo binding sites (RBSs) are located within the 4.3 kb repo cis-regulatory DNA (CRD). Using both S2 cell culture and in vivo expression systems, I have evidence that suggests Repo protein interacts strongly with one of these sites, designated RBS1, to induce the expression of reporter genes. Mutagenesis of RBS1 results in a significant decrease of reporter gene expression in both systems, while RBS2 and RBS3 appear to have no role in autoregulation of repo expression."],"dc:identifier":["https://egrove.olemiss.edu/etd/1355"],"dc:subject":["autoregulation","Drosophila","gcm","glia","repo","Biology"],"dc:title":["Autoregulation of the Glial Gene reversed polarity in Drosophila melanogaster"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D. in Biological Science"]},"updated_at":"2026-07-24T03:06:44Z"}