QUALITATIVE ANALYSIS OF LIPOPROTEINS AND STUDY OF THE METABOLIC PROFILE IN DIFFERENT CLINICAL SETTINGS BY RAMAN SPECTROSCOPY
type: CreativeWork
description: Raman spectroscopy (RS) is a promising optical technique, able to offer a rapid specific overview of the biochemical composition of the analyzed sample without any preparation or labeling. This non-invasive technique is based on the radiation/matter interaction where a sample is illuminated by a laser which interacts with the vibrational motions of the molecules, leading to the emission of light at wavelengths different from the incident one. This property is used to obtain a spectrum characterized by peaks referable to the biochemical composition of the sample analyzed. This approach has been integrated with conventional biochemical assay to study metabolic disorders such as: obesity (Ob) and type 2 diabetes mellitus (T2DM). RS was used to study the metabolic/biochemical profile of obese diabetic patients undergoing bariatric surgery (BS), to evaluate the impact of the surgery on body composition and systemic metabolism. The results obtained showed that, despite the improvement of the clinical parameters and levels of adipokines, ghrelin and FGF-19 dosed, Raman data identified a persistent alteration of the patients’ biochemical profile in the post-operative follow-up period (6-12 months), underlining the need for more extended longitudinal studies to assess the powerful of RS to monitor the progression of the disease. This method has also been used to study the quality of lipoproteins (LPs), multimolecular complexes responsible for the transport of lipids in the body. Currently, the amount of cholesterol carried by LPs is one of the main parameters used in clinical practice to assess the risk of cardiovascular events. In particular, the level of circulating low-density lipoprotein cholesterol (LDL-C) is considered one of the main risk factors for cardiovascular disease (CVD). It can deposit on the wall of the arteries, causing the formation and progression of atherosclerotic plaques. Conversely, elevated levels of circulating high-density lipoprotein (HDL-C) chole…
abstract: Raman spectroscopy (RS) is a promising optical technique, able to offer a rapid specific overview of the biochemical composition of the analyzed sample without any preparation or labeling. This non-invasive technique is based on the radiation/matter interaction where a sample is illuminated by a laser which interacts with the vibrational motions of the molecules, leading to the emission of light at wavelengths different from the incident one. This property is used to obtain a spectrum characterized by peaks referable to the biochemical composition of the sample analyzed. This approach has been integrated with conventional biochemical assay to study metabolic disorders such as: obesity (Ob) and type 2 diabetes mellitus (T2DM). RS was used to study the metabolic/biochemical profile of obese diabetic patients undergoing bariatric surgery (BS), to evaluate the impact of the surgery on body composition and systemic metabolism. The results obtained showed that, despite the improvement of the clinical parameters and levels of adipokines, ghrelin and FGF-19 dosed, Raman data identified a persistent alteration of the patients’ biochemical profile in the post-operative follow-up period (6-12 months), underlining the need for more extended longitudinal studies to assess the powerful of RS to monitor the progression of the disease. This method has also been used to study the quality of lipoproteins (LPs), multimolecular complexes responsible for the transport of lipids in the body. Currently, the amount of cholesterol carried by LPs is one of the main parameters used in clinical practice to assess the risk of cardiovascular events. In particular, the level of circulating low-density lipoprotein cholesterol (LDL-C) is considered one of the main risk factors for cardiovascular disease (CVD). It can deposit on the wall of the arteries, causing the formation and progression of atherosclerotic plaques. Conversely, elevated levels of circulating high-density lipoprotein (HDL-C) chole…
author: BONIZZI, ARIANNA
language: eng
keywords: Raman spectroscopy, obesity, type 2 diabetes mellitu, bariatric surgery, lipoprotein, quality, Settore BIO/10 - Biochimica, Settore MED/18 - Chirurgia Generale
date published: 2023-03-30
date modified: 2026-07-27T20:18:58Z
provider: Università degli Studi di Milano
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Università degli Studi di Milano

PropertyValue
property ID: Handle
value: https://hdl.handle.net/2434/959236

PropertyValue
property ID: DOI
value: 10.13130/bonizzi-arianna_phd2023-03-30