IMPORTANCE OF SUPPLYING DAIRY COWS WITH ESSENTIAL FATTY ACIDS (EFA) AND CONJUGATED LINOLEIC ACIDS (CLA) DURING THE TRANSITION PERIOD ON METABOLISM AND HEALTH
type: CreativeWork
description: Ketogenesis and steatosis are common sequelae of the severe mobilization of fat reserves necessary at the onset of lactation when the energy expenditures for milk and maintenance exceed the energy provided by voluntary feed intake in dairy cows. This situation along with a common state of systemic inflammation during early lactation makes dairy cow vulnerable to further metabolic and infectious diseases which impact their profitability. A periparturient supplementation with essential fatty acids (EFA) and conjugated linoleic acids (CLA) has been shown to influence different aspects of lipid and energy metabolisms as well as markers of immune homeostasis in dairy cows. This study aimed at investigating the synergistic effects of EFA, particularly α-linolenic acid (ALA, n-3 fatty acid (FA), and CLA on the liver and plasma proteome profile of dairy cows during the time spanning 3 weeks before to 9 weeks after parturition. Late-gestation Holstein cows were infused from 9 wk ante partum (AP) to 9 wk post partum (PP) into the abomasum with either coconut oil (CTRL, n = 8, 38 & 76 g/d during dry period and lactation, respectively) or a mixture of EFA (Linseed + safflower oil) and CLA (Lutalin, BASF) (EFA+CLA, n = 8, 60 and 120 g/d during dry period and lactation, respectively). An untargeted shotgun proteomics approach based on liquid chromatography coupled with tandem mass spectrometry was performed in liver biopsies and plasma samples collected at days -21, +1, +28, and +63 relative to calving. At each timepoint, differentially abundant proteins (DAP) between treatment groups were identified as the proteins at the intersection between a multivariate supervised Partial Least Squares Discriminant Analysis (PLS-DA) and an univariate analysis (proteins with P-value <0.05 according to a t-test and fold change ≥ 1.3). In liver a total of 1680 proteins was identified at each timepoint, of which 100 DAP between groups were assigned to the metabolism of xenobiotics by cytochrome …
abstract: Ketogenesis and steatosis are common sequelae of the severe mobilization of fat reserves necessary at the onset of lactation when the energy expenditures for milk and maintenance exceed the energy provided by voluntary feed intake in dairy cows. This situation along with a common state of systemic inflammation during early lactation makes dairy cow vulnerable to further metabolic and infectious diseases which impact their profitability. A periparturient supplementation with essential fatty acids (EFA) and conjugated linoleic acids (CLA) has been shown to influence different aspects of lipid and energy metabolisms as well as markers of immune homeostasis in dairy cows. This study aimed at investigating the synergistic effects of EFA, particularly α-linolenic acid (ALA, n-3 fatty acid (FA), and CLA on the liver and plasma proteome profile of dairy cows during the time spanning 3 weeks before to 9 weeks after parturition. Late-gestation Holstein cows were infused from 9 wk ante partum (AP) to 9 wk post partum (PP) into the abomasum with either coconut oil (CTRL, n = 8, 38 & 76 g/d during dry period and lactation, respectively) or a mixture of EFA (Linseed + safflower oil) and CLA (Lutalin, BASF) (EFA+CLA, n = 8, 60 and 120 g/d during dry period and lactation, respectively). An untargeted shotgun proteomics approach based on liquid chromatography coupled with tandem mass spectrometry was performed in liver biopsies and plasma samples collected at days -21, +1, +28, and +63 relative to calving. At each timepoint, differentially abundant proteins (DAP) between treatment groups were identified as the proteins at the intersection between a multivariate supervised Partial Least Squares Discriminant Analysis (PLS-DA) and an univariate analysis (proteins with P-value <0.05 according to a t-test and fold change ≥ 1.3). In liver a total of 1680 proteins was identified at each timepoint, of which 100 DAP between groups were assigned to the metabolism of xenobiotics by cytochrome …
author: VESHKINI, ARASH
language: eng
keywords: Fatty acid, lactation, metabolic adaptation, immunity, energy balance, Protein, Settore AGR/18 - Nutrizione e Alimentazione Animale
date published: 2022-05-25
date modified: 2026-07-27T20:19:08Z
provider: Università degli Studi di Milano
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Università degli Studi di Milano

PropertyValue
property ID: Handle
value: https://hdl.handle.net/2434/945231

PropertyValue
property ID: DOI
value: 10.13130/veshkini-arash_phd2022-05-25