CHROMOSOME AND GENOTYPE CHARACTERIZATION IN SOME RICE BIOTYPES SHOWING GRAIN SHATTERING
type: CreativeWork
description: Red rice (Oryza sativa var. silvatica L.) is a common weed in rice crop, very troublesome to control due to high shattering. The aim of the present research was to analyse the genome of some biotypes of red rice in order to promote innovatives methods for its control. In the first year, morphological and karyotypical analysis of some phenotypically different red rice biotypes in comparison with the rice cultivar ‘Loto’ were carried out. The seeds of rice and red rice were sown in greenhouse and afterwards the root tips of the seedlings were employed for the cytological analysis. The chromosome preparations were made by the standard air-drying technique, after enzymatic maceration, and stained with a 4% Giemsa solution for 20 min., according to Fukui and Iijima’s method (1992). The analysis, by means of a computerized chromosome image method (CHIA–EA), showed that in red rice various translocations occur, which always involve a chromosome of the first pair together with other elements of the set. Previous results clearly indicated that each red rice biotype is characterized by a specific translocation, showing a relationship among different phenotypes and karyotypes. It is important to note that in Oryza sativa one gene for shattering maps to the long arm of chromosome 1, which is always involved in the translocations. The project is divided into three phases: the field growing and observations, the laboratory analysis and the computer analysis. The first results that we have obtained support the proposed assumption. An increase in the dose of the genes frequently alters the phenotype, depending upon the effect of the genes involved. In the second year, the research was based on the molecular analysis. Since the shattering gene is located on the long arm of the chromosome 1, which is involved in the translocation process, I have analyzed this genomic locus by both cytogenetic and molecular biology techniques. The shattering character relies on the presence of a singl…
abstract: Red rice (Oryza sativa var. silvatica L.) is a common weed in rice crop, very troublesome to control due to high shattering. The aim of the present research was to analyse the genome of some biotypes of red rice in order to promote innovatives methods for its control. In the first year, morphological and karyotypical analysis of some phenotypically different red rice biotypes in comparison with the rice cultivar ‘Loto’ were carried out. The seeds of rice and red rice were sown in greenhouse and afterwards the root tips of the seedlings were employed for the cytological analysis. The chromosome preparations were made by the standard air-drying technique, after enzymatic maceration, and stained with a 4% Giemsa solution for 20 min., according to Fukui and Iijima’s method (1992). The analysis, by means of a computerized chromosome image method (CHIA–EA), showed that in red rice various translocations occur, which always involve a chromosome of the first pair together with other elements of the set. Previous results clearly indicated that each red rice biotype is characterized by a specific translocation, showing a relationship among different phenotypes and karyotypes. It is important to note that in Oryza sativa one gene for shattering maps to the long arm of chromosome 1, which is always involved in the translocations. The project is divided into three phases: the field growing and observations, the laboratory analysis and the computer analysis. The first results that we have obtained support the proposed assumption. An increase in the dose of the genes frequently alters the phenotype, depending upon the effect of the genes involved. In the second year, the research was based on the molecular analysis. Since the shattering gene is located on the long arm of the chromosome 1, which is involved in the translocation process, I have analyzed this genomic locus by both cytogenetic and molecular biology techniques. The shattering character relies on the presence of a singl…
author: C. Ruini
language: eng
keywords: Red rice, Shattering, Translocation, Settore AGR/02 - Agronomia e Coltivazioni Erbacee, Settore AGR/04 - Orticoltura e Floricoltura, Settore AGR/07 - Genetica Agraria, Settore AGR/17 - Zootecnica Generale e Miglioramento Genetico
date published: 2010-12-16
date modified: 2026-07-27T20:19:17Z
provider: Università degli Studi di Milano
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Università degli Studi di Milano

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2434/150217

PropertyValue
property ID: DOI
value: 10.13130/ruini-chiara_phd2010-12-16