{"id":{"repo_id":"milano","oai_identifier":"oai:air.unimi.it:2434/1205381"},"canonical_url":"https://search.dev.ndltd.org/etd/milano/oai:air.unimi.it:2434/1205381","repository":{"repo_id":"milano","name":"Università degli Studi di Milano","base_url":"https://air.unimi.it/oai/request"},"display":{"title":"FROM AGRI-FOOD WASTE TO HIGH-VALUE COMPOUNDS VIA GREEN TECHNOLOGY APPROACHES","abstract":"In the framework of sustainable biocatalysis and circular bioeconomy, this thesis explores the development of innova:ve enzyma:c strategies for the valoriza:on of agri-food by- products into high-value compounds. Four complementary approaches were pursued, each focusing on process intensifica:on, enzyme engineering, and biocatalyst immobiliza:on to maximize efficiency, scalability, and environmental sustainability. First, a co-immobilized mul:-enzyme system combining a commercial α-rhamnosidase and an extremophilic β-glycosidase was implemented for the efficient hydrolysis of citrus ru:nosyl flavonoids, achieving >99% conversion in con:nuous flow under zero-waste condi:ons. Second, soybean glycosides were converted into bioac:ve aglycones and lipophilized deriva:ves through a dual strategy involving hydroly:c and esterifica:on reac:ons under flow, thus improving their physicochemical proper:es for applica:ons in food, pharmaceu:cal, and cosme:c sectors. Third, an integrated process was designed for the recovery of phlore:n from apple pomace, employing an extremophilic glycosidase immobilized on bacterial cellulose films, where both substrate and support were derived from the same biomass, reinforcing the concept of feedstock circularity. Finally, a self-sufficient biocatalyst combining an ene reductase and a glucose dehydrogenase was developed for the selec:ve bioreduc:on of cinnamaldehyde, achieving high conversion and opera:onal stability under con:nuous flow, with in situ NADH regenera:on. Overall, this research provides new insights into the exploita:on of extremophilic enzymes, immobiliza:on strategies, and flow biocatalysis for the sustainable produc:on of bioac:ve molecules, highligh:ng their poten:al industrial relevance in the food, cosme:c, and pharmaceu:cal sectors.","abstract_html":"In the framework of sustainable biocatalysis and circular bioeconomy, this thesis explores the development of innova:ve enzyma:c strategies for the valoriza:on of agri-food by- products into high-value compounds. Four complementary approaches were pursued, each focusing on process intensifica:on, enzyme engineering, and biocatalyst immobiliza:on to maximize efficiency, scalability, and environmental sustainability. First, a co-immobilized mul:-enzyme system combining a commercial α-rhamnosidase and an extremophilic β-glycosidase was implemented for the efficient hydrolysis of citrus ru:nosyl flavonoids, achieving >99% conversion in con:nuous flow under zero-waste condi:ons. Second, soybean glycosides were converted into bioac:ve aglycones and lipophilized deriva:ves through a dual strategy involving hydroly:c and esterifica:on reac:ons under flow, thus improving their physicochemical proper:es for applica:ons in food, pharmaceu:cal, and cosme:c sectors. Third, an integrated process was designed for the recovery of phlore:n from apple pomace, employing an extremophilic glycosidase immobilized on bacterial cellulose films, where both substrate and support were derived from the same biomass, reinforcing the concept of feedstock circularity. Finally, a self-sufficient biocatalyst combining an ene reductase and a glucose dehydrogenase was developed for the selec:ve bioreduc:on of cinnamaldehyde, achieving high conversion and opera:onal stability under con:nuous flow, with in situ NADH regenera:on. Overall, this research provides new insights into the exploita:on of extremophilic enzymes, immobiliza:on strategies, and flow biocatalysis for the sustainable produc:on of bioac:ve molecules, highligh:ng their poten:al industrial relevance in the food, cosme:c, and pharmaceu:cal sectors.","abstract_has_math":false,"creators":["COLACICCO, AGOSTINA"],"institution":"Università degli Studi di Milano","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["advisor: M. L. CONTENTE ; co-tutor: F. MOLINARI ; Ph.D. Dean: D. MORA","A. Colacicco","CONTENTE, MARTINA LETIZIA","MORA, DIEGO"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-12-19","date_published":"2025-12-19","updated_at":"2026-07-27T20:18:43Z","subjects":["Settore CHEM-07/B - Chimica degli alimenti"],"languages":["eng"],"rights":["info:eu-repo/semantics/embargoedAccess","license:Creative commons","license uri:http://creativecommons.org/licenses/by-sa/4.0/"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2434/1205381","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["advisor: M. L. CONTENTE ; co-tutor: F. MOLINARI ; Ph.D. Dean: D. MORA","A. 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Four complementary approaches were pursued, each focusing on process intensifica:on, enzyme engineering, and biocatalyst immobiliza:on to maximize efficiency, scalability, and environmental sustainability. First, a co-immobilized mul:-enzyme system combining a commercial α-rhamnosidase and an extremophilic β-glycosidase was implemented for the efficient hydrolysis of citrus ru:nosyl flavonoids, achieving >99% conversion in con:nuous flow under zero-waste condi:ons. Second, soybean glycosides were converted into bioac:ve aglycones and lipophilized deriva:ves through a dual strategy involving hydroly:c and esterifica:on reac:ons under flow, thus improving their physicochemical proper:es for applica:ons in food, pharmaceu:cal, and cosme:c sectors. Third, an integrated process was designed for the recovery of phlore:n from apple pomace, employing an extremophilic glycosidase immobilized on bacterial cellulose films, where both substrate and support were derived from the same biomass, reinforcing the concept of feedstock circularity. Finally, a self-sufficient biocatalyst combining an ene reductase and a glucose dehydrogenase was developed for the selec:ve bioreduc:on of cinnamaldehyde, achieving high conversion and opera:onal stability under con:nuous flow, with in situ NADH regenera:on. Overall, this research provides new insights into the exploita:on of extremophilic enzymes, immobiliza:on strategies, and flow biocatalysis for the sustainable produc:on of bioac:ve molecules, highligh:ng their poten:al industrial relevance in the food, cosme:c, and pharmaceu:cal sectors."]},{"key":"dc:title","label":"Title","values":["FROM AGRI-FOOD WASTE TO HIGH-VALUE COMPOUNDS VIA GREEN TECHNOLOGY APPROACHES"]}]}],"canonical_facts":{"dc:contributor":["advisor: M. L. CONTENTE ; co-tutor: F. MOLINARI ; Ph.D. Dean: D. MORA","A. Colacicco","CONTENTE, MARTINA LETIZIA","MORA, DIEGO"],"dc:creator":["COLACICCO, AGOSTINA"],"dc:date":["2025-12-19"],"dc:description":["In the framework of sustainable biocatalysis and circular bioeconomy, this thesis explores the development of innova:ve enzyma:c strategies for the valoriza:on of agri-food by- products into high-value compounds. Four complementary approaches were pursued, each focusing on process intensifica:on, enzyme engineering, and biocatalyst immobiliza:on to maximize efficiency, scalability, and environmental sustainability. First, a co-immobilized mul:-enzyme system combining a commercial α-rhamnosidase and an extremophilic β-glycosidase was implemented for the efficient hydrolysis of citrus ru:nosyl flavonoids, achieving >99% conversion in con:nuous flow under zero-waste condi:ons. Second, soybean glycosides were converted into bioac:ve aglycones and lipophilized deriva:ves through a dual strategy involving hydroly:c and esterifica:on reac:ons under flow, thus improving their physicochemical proper:es for applica:ons in food, pharmaceu:cal, and cosme:c sectors. Third, an integrated process was designed for the recovery of phlore:n from apple pomace, employing an extremophilic glycosidase immobilized on bacterial cellulose films, where both substrate and support were derived from the same biomass, reinforcing the concept of feedstock circularity. Finally, a self-sufficient biocatalyst combining an ene reductase and a glucose dehydrogenase was developed for the selec:ve bioreduc:on of cinnamaldehyde, achieving high conversion and opera:onal stability under con:nuous flow, with in situ NADH regenera:on. Overall, this research provides new insights into the exploita:on of extremophilic enzymes, immobiliza:on strategies, and flow biocatalysis for the sustainable produc:on of bioac:ve molecules, highligh:ng their poten:al industrial relevance in the food, cosme:c, and pharmaceu:cal sectors."],"dc:identifier":["https://hdl.handle.net/2434/1205381"],"dc:language":["eng"],"dc:publisher":["Università degli Studi di Milano"],"dc:relation":["numberofpages:181"],"dc:rights":["info:eu-repo/semantics/embargoedAccess","license:Creative commons","license uri:http://creativecommons.org/licenses/by-sa/4.0/"],"dc:subject":["Settore CHEM-07/B - Chimica degli alimenti"],"dc:title":["FROM AGRI-FOOD WASTE TO HIGH-VALUE COMPOUNDS VIA GREEN TECHNOLOGY APPROACHES"],"dc:type":["info:eu-repo/semantics/doctoralThesis"]},"updated_at":"2026-07-27T20:18:43Z"}