BACTERIAL PLAYERS IN CROP SYSTEMS: BENEFITS AND RISKS IN THE " ONE HEALTH" PERSPECTIVES.
type: CreativeWork
description: In recent years, agri-food ecosystems have recognized as crucial environment in the One Health approach, which deals with the intersections between human, animal and environmental health, recognising them as a unique interconnected system. The research conducted in the frame of this PhD thesis dealt with the investigation on the effect of bacteria entrance in agri-food ecosystems. In compliance with the ‘One Health’ concept, both the benefits and the risks given by the introduction, voluntary or accidental, of bacterial players in environmental habitats related to food production were considered. An example of deliberate release of microbes in agricultural systems is the use of plant beneficial strains (i.e., biofertilizer and biostimulants). Experiments were designed to better clarify the interactions occurring between bacteria inoculants and the recipient plant microbiome and, additionally, the research was focused on the design of novel delivery system for the administration of plant probiotics. The knowledge improvement regarding these two aspects has the potential to promote the transition from the lab to the field of microbial based solution for sustainable agriculture, such as the replacement of chemical fertilizers or the use of strains to improve plant tolerance to biotic and abiotic stresses. We characterized for Plant Growth Promotion (PGP) activities a collection of endophytic strains established from grapevine and lettuce plants collected in the field. Among the most promising strains, Rhizobium sp. GR12 and Kosakonia sp. VR04 were tagged with genes coding for fluorescent proteins and used to inoculate micropropagated grapevine cuttings. Plantlets were grown in vitro under optimal growth conditions or on a diluted medium to mimic nutritional deficit. The measurement of plant biomass showed that Rhizobium sp. GR12 displayed PGP activity, when the plantlest were grown under nutritional deficit. The colonization of the plant tissues was confirmed for both …
abstract: In recent years, agri-food ecosystems have recognized as crucial environment in the One Health approach, which deals with the intersections between human, animal and environmental health, recognising them as a unique interconnected system. The research conducted in the frame of this PhD thesis dealt with the investigation on the effect of bacteria entrance in agri-food ecosystems. In compliance with the ‘One Health’ concept, both the benefits and the risks given by the introduction, voluntary or accidental, of bacterial players in environmental habitats related to food production were considered. An example of deliberate release of microbes in agricultural systems is the use of plant beneficial strains (i.e., biofertilizer and biostimulants). Experiments were designed to better clarify the interactions occurring between bacteria inoculants and the recipient plant microbiome and, additionally, the research was focused on the design of novel delivery system for the administration of plant probiotics. The knowledge improvement regarding these two aspects has the potential to promote the transition from the lab to the field of microbial based solution for sustainable agriculture, such as the replacement of chemical fertilizers or the use of strains to improve plant tolerance to biotic and abiotic stresses. We characterized for Plant Growth Promotion (PGP) activities a collection of endophytic strains established from grapevine and lettuce plants collected in the field. Among the most promising strains, Rhizobium sp. GR12 and Kosakonia sp. VR04 were tagged with genes coding for fluorescent proteins and used to inoculate micropropagated grapevine cuttings. Plantlets were grown in vitro under optimal growth conditions or on a diluted medium to mimic nutritional deficit. The measurement of plant biomass showed that Rhizobium sp. GR12 displayed PGP activity, when the plantlest were grown under nutritional deficit. The colonization of the plant tissues was confirmed for both …
author: PATANIA, GIOVANNI
language: eng
keywords: Settore AGRI-08/A - Microbiologia agraria, alimentare e ambientale
date published: 2024-12-18
date modified: 2026-07-27T20:19:12Z
provider: Università degli Studi di Milano
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Università degli Studi di Milano

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property ID: Handle
value: https://hdl.handle.net/2434/1118548